8X5M Chain A
Mitogen-activated protein kinase 8 (MAPK8)
Inactive — 0.0%DFG-inαC-inType1 · Y4O
Resolution
2.0 Å
R-value
0.195
Predicted activity confidence0.0%
Structure info
Alternate conformationA
Missing atoms0
Missing residues4
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
DNNFYSVEIGDSTFTVLKRYQNLKPIGGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDTLKILDFGLARVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMIKGGVLFPGTDHIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDL
UniProt reference sequence
YQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYI
Aligned reference sequence
YQNL----------------KPIGSGA----------------QGIVCAAYD--AI------------------------------------LERNVAIKKLS--RPFQNQTH--------------------------AKRAYRELVLMKCV--------------------------NHKNIIGLLNVFTPQK--SLEEFQ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYIVMELMD---------------ANLCQVIQM-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELDHERMSYLLYQMLCGIKHLHSA---------------------------------------------GIIHRDL-----KPSNIVVKS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCTLKILDFGLARTAGTSFMM------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TPYVVTRYYRAPEVILG--------------------------------MGYK-ENVDLWSVGCIMGEMVCH-----------------KILFPG--------------------------------------------------RDYIDQWNKVIEQ--LGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKA------------------------------------------------------------SQARDLLSKMLVIDASKRIS--------------VDEALQHPYI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YQNL----------------KPIG--------------------GIVCAAYD--AI------------------------------------LERNVAIKKLS--RPFQNQTH--------------------------AKRAYRELVLMKCV--------------------------NHKNIIGLLNVFTPQK--SLEEFQ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYIVMELMD---------------ANLCQVIQM-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELDHERMSYLLYQMLCGIKHLHSA---------------------------------------------GIIHRDL-----KPSNIVVKS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D-TLKILDFGLAR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VTRYYRAPEVILG--------------------------------MGYK-ENVDIWSVGCIMGEMIKG-----------------GVLFPG--------------------------------------------------TDHIDQWNKVIEQ--LGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKA------------------------------------------------------------SQARDLLSKMLVIDASKRIS--------------VDEALQHPYI
Activation segment
DFGLAR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VTRYYRAPE
Binding pocket
KPIG____GIVCAVAIKKLRAYRELVLMKCVNKNIIGLLNVYIVMELMD_ANLCQVIQMHLHSAGIIHRDLKPSNIVVILDFGLA
Ligand info
Orthosteric ligand
Y4O
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 8X5M, Chain A