8WSW Chain A
LIM domain kinase 2 (LIMK2)
Inactive — 1.1%DFG-inαC-outType1 · LH0
Resolution
2.5 Å
R-value
0.212
Predicted activity confidence1.1%
Structure info
Alternate conformationB
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
DLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGL
UniProt reference sequence
LIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEA
Aligned reference sequence
LIHG----------------EVLGKGF----------------FGQAIKVTH--KA------------------------------------TGKVMVMKELI--RCDEET----------------------------QKTFLTEVKVMRSL--------------------------DHPNVLKFIGVLYKDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLNLLTEYIEG--------------GTLKDFLRS--MD-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFPWQQKVRFAKGIASGMAYLHSM---------------------------------------------CIIHRDL-----NSHNCLIKL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKTVVVADFGLSRLIVEERKR--APMEKATTKKRTLRKNDRKKR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVVGNPYWMAPEMLNG--------------------------------KSYD-ETVDIFSFGIVLCEIIGQ-------------------------VYADPDCLPRTL------------------------------------DFGLNVKLFWEKF--VPTDCP---------------------------------------------------------------------------------------------------------PAFFPLAAICCRLEPESRPA--------------FSKLEDSFEA
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LIHG----------------EVLGKGF----------------FGQAIKVTH--KA------------------------------------TGKVMVMKELI--RCDEET----------------------------QKTFLTEVKVMRSL--------------------------DHPNVLKFIGVLYKDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLNLLTEYIEG--------------GTLKDFLRS--MD-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFPWQQKVRFAKGIASGMAYLHSM---------------------------------------------CIIHRDL-----NSHNCLIKL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKTVVVADFGLSRLIVE------------------------KKR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVVGNPYWMAPEMLNG--------------------------------KSYD-ETVDIFSFGIVLCEIIGQ-------------------------VYADPDCLPRTL------------------------------------DFGLNVKLFWEKF--VPTDCP---------------------------------------------------------------------------------------------------------PAFFPLAAICCRLEPESRPA--------------FSKLEDSFEA
Activation segment
DFGLSRLIVE------------------------KKR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVVGNPYWMAPE
Binding pocket
EVLGKGFFGQAIKMVMKELTFLTEVKVMRSLDPNVLKFIGVNLLTEYIEGGTLKDFLRSYLHSMCIIHRDLNSHNCLIVADFGLS
Ligand info
Orthosteric ligand
LH0
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 8WSW, Chain A