8UAP Chain A
G protein-coupled receptor kinase 5 (GRK5)
Active — 100.0%DFG-inαC-inType1 · W2T
Resolution
2.5 Å
R-value
0.229
Predicted activity confidence100.0%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
GKSKKWKEILKFPHISQCEDLRRTIDRDYCSLCDKQPIGRLLFRQFCETRPGLECYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEEKLLQKPCKELFSACAQSVHEYLRGEPFHEYLDSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRNLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHP
UniProt reference sequence
FRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFF
Aligned reference sequence
FRQY----------------RVLGKGG----------------FGEVCACQV--RA------------------------------------TGKMYACKRLE--KKRIKKRKG-------------------------ESMALNEKQILEKV--------------------------NSQFVVNLAYAYETKD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALCLVLTIMNG--------------GDLKFHIYN--MGNP-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GFEEERALFYAAEILCGLEDLHRE---------------------------------------------NTVYRDL-----KPENILLDD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YGHIRISDLGLAVKIPEGDLI------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGRVGTVGYMAPEVLNN--------------------------------QRYG-LSPDYWGLGCLIYEMIEG-----------------QSPFRG--RKEK--------------------------------------------VKREEVDRRVLET--EEVYSHKFS------------------------------------------------------------------------------------------------------EEAKSICKMLLTKDAKQRLG--CQEEG-------AAEVKRHPFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FRQY----------------RVLGKGG----------------FGEVCACQV--RA------------------------------------TGKMYACKRLE--KKRIKKRKG-------------------------ESMALNEKQILEKV--------------------------NSQFVVNLAYAYETKD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALCLVLTIMNG--------------GDLKFHIYN--MGNP-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GFEEERALFYAAEILCGLEDLHRE---------------------------------------------NTVYRNL-----KPENILLDD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YGHIRISDLGLAVKIPEGDLI------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGRVGTVGYMAPEVLNN--------------------------------QRYG-LSPDYWGLGCLIYEMIEG-----------------QSPFRG--RKEK--------------------------------------------VKREEVDRRVLET--EEVYSHKFS------------------------------------------------------------------------------------------------------EEAKSICKMLLTKDAKQRLG--CQEEG-------AAEVKRHPFF
Activation segment
DLGLAVKIPEGDLI------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGRVGTVGYMAPE
Binding pocket
RVLGKGGFGEVCAYACKRLMALNEKQILEKVNQFVVNLAYACLVLTIMNGGDLKFHIYNDLHRENTVYRNLKPENILLISDLGLA
Ligand info
Orthosteric ligand
W2T
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 8UAP, Chain A