8R4Q Chain A
Serine/threonine-protein kinase SIK3 (SIK3)
Active — 84.7%DFG-inαC-inType1 · DB8
Resolution
2.838 Å
R-value
0.214
Predicted activity confidence84.7%
Structure info
Alternate conformation—
Missing atoms4
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
RIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKMLSHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHSRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLKWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICKHKWMKLGDADPNFDRLIAESQQPLNEDVLLAMEDMGLDKEQTLQSLRSDAYDHYSAIYSLLCD
UniProt reference sequence
YEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICKHKWM
Aligned reference sequence
YEID----------------RTIGKGN----------------FAVVKRATH--LV------------------------------------TKAKVAIKIID--KTQLDEEN--------------------------LKKIFREVQIMKML--------------------------CHPHIIRLYQVMETER--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MIYLVTEYASG--------------GEIFDHLVA--HG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMAEKEARRKFKQIVTAVYFCHCR---------------------------------------------NIVHRDL-----KAENLLLDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLNIKIADFGFSNLFTPGQLL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTWCGSPPYAAPELFEG--------------------------------KEYDGPKVDIWSLGVVLYVLVCG-----------------ALPFDG--------------------------------------------------STLQNLRARVLSG--KFRIPFFMS------------------------------------------------------------------------------------------------------TECEHLIRHMLVLDPNKRLS--------------MEQICKHKWM
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YEID----------------RTIGKGN----------------FAVVKRATH--LV------------------------------------TKAKVAIKIID--KTQLDEEN--------------------------LKKIFREVQIMKML--------------------------SHPHIIRLYQVMETER--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MIYLVTEYASG--------------GEIFDHLVA--HG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMAEKEARRKFKQIVTAVYFCHSR---------------------------------------------NIVHRDL-----KAENLLLDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLNIKIADFGFSNLFTPGQLL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K-WCGSPPYAAPELFEG--------------------------------KEYDGPKVDIWSLGVVLYVLVCG-----------------ALPFDG--------------------------------------------------STLQNLRARVLSG--KFRIPFFMS------------------------------------------------------------------------------------------------------TECEHLIRHMLVLDPNKRLS--------------MEQICKHKWM
Activation segment
DFGFSNLFTPGQLL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K-WCGSPPYAAPE
Binding pocket
RTIGKGNFAVVKRVAIKIIKIFREVQIMKMLSPHIIRLYQVYLVTEYASGGEIFDHLVAFCHSRNIVHRDLKAENLLLIADFGFS
Ligand info
Orthosteric ligand
DB8
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 8R4Q, Chain A