8GMD Chain A
AP2-associated protein kinase 1 (AAK1)
Active — 98.1%DFG-inαC-inType1 · ZRR
Resolution
2.2 Å
R-value
0.233
Predicted activity confidence98.1%
Structure info
Alternate conformation—
Missing atoms57
Missing residues1
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
YIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPI
UniProt reference sequence
VTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFK
Aligned reference sequence
VTVD----------------EVLAEGG----------------FAIVFLVRT--S-------------------------------------NGMKCALKRMF--VNNEHD----------------------------LQVCKREIQIMRDL--S-----------------------GHKNIVGYIDSSINNV--SSGDVW------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EVLILMDFCRG--------------GQVVNLMNQ--RLQT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GFTENEVLQIFCDTCEAVARLHQC--KT-----------------------------------------PIIHRDL-----KVENILLHD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGHYVLCDFGSATNKFQNPQT--EGVNAVEDE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IKKYTTLSYRAPEMVNL--YSG---------------------------KIIT-TKADIWALGCLLYKLCYF-----------------TLPFGE-------------------------------------------------------SQVAICDG--NFTIPDNSRYS----------------------------------------------------------------------------------------------------QDMHCLIRYMLEPDPDKRPD--------------IYQVSYFSFK
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
VTVD----------------EVLAEGG----------------FAIVFLVRT--S-------------------------------------NGMKCALKRMF--VNNEHD----------------------------LQVCKREIQIMRDL--------------------------GHKNIVGYIDSSINNV--SSGDVW------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EVLILMDFCRG--------------GQVVNLMNQ--RLQT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GFTENEVLQIFCDTCEAVARLHQC--KT-----------------------------------------PIIHRDL-----KVENILLHD---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GHYVLCDFGSATNKFQNPQT--EGVNAVEDE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IKKYTTLSYRAPEMVNL--YSG---------------------------KIIT-TKADIWALGCLLYKLCYF-----------------TLPFGE-------------------------------------------------------SQVAICDG--NFTIPDNSRYS----------------------------------------------------------------------------------------------------QDMHCLIRYMLEPDPDKRPD--------------IYQVSYFSFK
Activation segment
DFGSATNKFQNPQT--EGVNAVEDE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IKKYTTLSYRAPE
Binding pocket
EVLAEGGFAIVFLCALKRMVCKREIQIMRDL_KNIVGYIDSLILMDFCRGGQVVNLMNQHQCKTPIIHRDLKVENILLLCDFGSA
Ligand info
Orthosteric ligand
ZRR
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 8GMD, Chain A