7Z4V Chain A
Serine/threonine-protein kinase 25 (STK25)
Active — 99.7%DFG-inαC-inType1 · IB5
Resolution
1.644 Å
R-value
0.247
Predicted activity confidence99.7%
Structure info
Alternate conformationB
Missing atoms0
Missing residues2
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
HLRGFANQHSRVDPEELFTKLDRIGSFGEVYKGIDNHTKEVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNFVGTPFWMAPEVIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNSPPTLEGQHSKPFKEFVEACLNKDPRFRPTAKELLKHKFITRYTKKTSFLTELIDRYKRWKSE
UniProt reference sequence
FTKLDRIGKGSFGEVYKGIDNHTKEVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNSPPTLEGQHSKPFKEFVEACLNKDPRFRPTAKELLKHKFI
Aligned reference sequence
FTKL----------------DRIGKGS----------------FGEVYKGID--NH------------------------------------TKEVVAIKIID--LEEAEDE---------------------------IEDIQQEITVLSQC--------------------------DSPYITRYFGSYLKST--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLWIIMEYLGG--------------GSALDLLKP--G--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLEETYIATILREILKGLDYLHSE---------------------------------------------RKIHRDI-----KAANVLLSE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGDVKLADFGVAGQLTDTQIK--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NTFVGTPFWMAPEVIKQ--------------------------------SAYD-FKADIWSLGITAIELAKG-----------------EPPNSD--------------------------------------------------LHPMRVLFLIPKN--SPPTLEGQHS-----------------------------------------------------------------------------------------------------KPFKEFVEACLNKDPRFRPT--------------AKELLKHKFI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FTKL----------------DRI--GS----------------FGEVYKGID--NH------------------------------------TKEVVAIKIID--LEEAEDE---------------------------IEDIQQEITVLSQC--------------------------DSPYITRYFGSYLKST--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLWIIMEYLGG--------------GSALDLLKP--G--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLEETYIATILREILKGLDYLHSE---------------------------------------------RKIHRDI-----KAANVLLSE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGDVKLADFGVAGQLTDTQIK--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------N-FVGTPFWMAPEVIKQ--------------------------------SAYD-FKADIWSLGITAIELAKG-----------------EPPNSD--------------------------------------------------LHPMRVLFLIPKN--SPPTLEGQHS-----------------------------------------------------------------------------------------------------KPFKEFVEACLNKDPRFRPT--------------AKELLKHKFI
Activation segment
DFGVAGQLTDTQIK--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------N-FVGTPFWMAPE
Binding pocket
DRIG__SFGEVYKVAIKIIDIQQEITVLSQCDPYITRYFGSWIIMEYLGGGSALDLLKPYLHSERKIHRDIKAANVLLLADFGVA
Ligand info
Orthosteric ligand
IB5
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 7Z4V, Chain A