7T4W Chain A
cGMP-dependent protein kinase 1 (PRKG1)
Active — 99.0%DFG-inαC-in
Resolution
2.23 Å
R-value
0.207
Predicted activity confidence99.0%
Structure info
Alternate conformationB
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
GLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF
UniProt reference sequence
FNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWF
Aligned reference sequence
FNII----------------DTLGVGG----------------FGRVELVQL--KSE-----------------------------------ESKTFAMKILK--KRHIVDTRQ-------------------------QEHIRSEKQIMQGA--------------------------HSDFIVRLYRTFKDSK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YLYMLMEACLG--------------GELWTILRD--RG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SFEDSTTRFYTACVVEAFAYLHSK---------------------------------------------GIIYRDL-----KPENLILDH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGYAKLVDFGFAKKIGFGKKT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WTFCGTPEYVAPEIILN--------------------------------KGHD-ISADYWSLGILMYELLTG-----------------SPPFSG--------------------------------------------------PDPMKTYNIILRG--IDMIEFPKKIA----------------------------------------------------------------------------------------------------KNAANLIKKLCRDNPSERLG--NLKNG-------VKDIQKHKWF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FNII----------------DTLGVGG----------------FGRVELVQL--KSE-----------------------------------ESKTFAMKILK--KRHIVDTRQ-------------------------QEHIRSEKQIMQGA--------------------------HSDFIVRLYRTFKDSK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YLYMLMEACLG--------------GELWTILRD--RG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SFEDSTTRFYTACVVEAFAYLHSK---------------------------------------------GIIYRDL-----KPENLILDH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGYAKLVDFGFAKKIGFGKKT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------W-FCGTPEYVAPEIILN--------------------------------KGHD-ISADYWSLGILMYELLTG-----------------SPPFSG--------------------------------------------------PDPMKTYNIILRG--IDMIEFPKKIA----------------------------------------------------------------------------------------------------KNAANLIKKLCRDNPSERLG--NLKNG-------VKDIQKHKWF
Activation segment
DFGFAKKIGFGKKT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------W-FCGTPEYVAPE
Binding pocket
DTLGVGGFGRVELFAMKILHIRSEKQIMQGAHDFIVRLYRTYMLMEACLGGELWTILRDYLHSKGIIYRDLKPENLILLVDFGFA
Ligand info
Orthosteric ligand
None
Allosteric ligand
EZJ
Ligand typeAllosteric
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 7T4W, Chain A