7NCF Chain A
Homeodomain-interacting protein kinase 2 (HIPK2)
Active — 98.7%DFG-inαC-inType1 · U82
Resolution
2.72 Å
R-value
0.197
Predicted activity confidence98.7%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
SMDYQLVQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVIDFGSASHVSKAVCSTLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDTDPYPLWRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLVEKADRREFIDLLKKMLTIDADKRITPIETLNHPFVTMTHLLDF
UniProt reference sequence
YEVLEFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLVEKADRREFIDLLKKMLTIDADKRITPIETLNHPFV
Aligned reference sequence
YEVL----------------EFLGRGT----------------FGQVVKCWK--RG------------------------------------TNEIVAIKILK--NHPSY-----------------------------ARQGQIEVSILARL--STESA-------------------DDYNFVRAYECFQHKN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HTCLVFEMLE---------------QNLYDFLKQ--NKFS-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLPLKYIRPVLQQVATALMKLKSL---------------------------------------------GLIHADL-----KPENIMLVD--PSRQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PYRVKVIDFGSASHVSKAVC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STYLQSRYYRAPEIILG--------------------------------LPFC-EAIDMWSLGCVIAELFLG-----------------WPLYPG--------------------------------------------------ASEYDQIRYISQT--QGLPAEYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLVEKADR------------------------------REFIDLLKKMLTIDADKRIT--------------PIETLNHPFV
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YEVL----------------EFLGRGT----------------FGQVVKCWK--RG------------------------------------TNEIVAIKILK--NHPSY-----------------------------ARQGQIEVSILARL--STESA-------------------DDYNFVRAYECFQHKN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HTCLVFEMLE---------------QNLYDFLKQ--NKFS-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLPLKYIRPVLQQVATALMKLKSL---------------------------------------------GLIHADL-----KPENIMLVD--PSRQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PYRVKVIDFGSASHVSKAVC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ST-LQSRYYRAPEIILG--------------------------------LPFC-EAIDMWSLGCVIAELFLG-----------------WPLYPG--------------------------------------------------ASEYDQIRYISQT--QGLPAEYLLSAGTKTTRFFNRDTD-PYPLWRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLVEKADR------------------------------REFIDLLKKMLTIDADKRIT--------------PIETLNHPFV
Activation segment
DFGSASHVSKAVC-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ST-LQSRYYRAPE
Binding pocket
EFLGRGTFGQVVKVAIKILQGQIEVSILARLSYNFVRAYECCLVFEMLE_QNLYDFLKQKLKSLGLIHADLKPENIMLVIDFGSA
Ligand info
Orthosteric ligand
U82
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 7NCF, Chain A