7KX8 Chain A
Serine/threonine-protein kinase DCLK1 (DCLK1)
Active — 97.8%DFG-inαC-inType1 · X8J
Resolution
3.1 Å
R-value
0.196
Predicted activity confidence97.8%
Structure info
Alternate conformation—
Missing atoms24
Missing residues4
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
IPATITERYKVGRTIGDGNFAVVKECVERSTAREYALKIIKKSKCREHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSQEVLFDQILMGQVDFPSPYWDNVSDSAKELITMMLLVDVDQRFSAVQVLEHPWVNHQLSVAGKIKKHF
UniProt reference sequence
YKVGRTIGDGNFAVVKECVERSTAREYALKIIKKSKCRGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELITMMLLVDVDQRFSAVQVLEHPWV
Aligned reference sequence
YKVG----------------RTIGDGN----------------FAVVKECVE--RS------------------------------------TAREYALKIIK--KSKCRGK---------------------------EHMIQNEVSILRRV--------------------------KHPNIVLLIEEMDVPT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELYLVMELVKG--------------GDLFDAITS--TN-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KYTERDASGMLYNLASAIKYLHSL---------------------------------------------NIVHRDI-----KPENLLVYE--HQDG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SKSLKLGDFGLATIVDGPL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVCGTPTYVAPEIIAE--------------------------------TGYG-LKVDIWAAGVITYILLCG-----------------FPPFRG--SG----------------------------------------------DDQEVLFDQILMG--QVDFPSPYWDNVS--------------------------------------------------------------------------------------------------DSAKELITMMLLVDVDQRFS--------------AVQVLEHPWV
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YKVG----------------RTIGDGN----------------FAVVKECVE--RS------------------------------------TAREYALKIIK--KSKCR-----------------------------EHMIQNEVSILRRV--------------------------KHPNIVLLIEEMDVPT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELYLVMELVKG--------------GDLFD----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTERDASGMLYNLASAIKYLHSL---------------------------------------------NIVHRDI-----KPENLLVYE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KSLKLGDFGLATIVDGPL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVCGTPTYVAPEIIAE--------------------------------TGYG-LKVDIWAAGVITYILLCG-----------------FPPFRG--S-------------------------------------------------QEVLFDQILMG--QVDFPSPYWDNVS--------------------------------------------------------------------------------------------------DSAKELITMMLLVDVDQRFS--------------AVQVLEHPWV
Activation segment
DFGLATIVDGPL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVCGTPTYVAPE
Binding pocket
RTIGDGNFAVVKEYALKIIMIQNEVSILRRVKPNIVLLIEEYLVMELVKGGDLFD____YLHSLNIVHRDIKPENLLVLGDFGLA
Ligand info
Orthosteric ligand
X8J
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 7KX8, Chain A