7ATU Chain A
LIM domain kinase 1 (LIMK1)
Inactive — 6.3%DFG-outαC-outType3 · RXN
Resolution
2.8 Å
R-value
0.244
Predicted activity confidence6.3%
Structure info
Alternate conformation—
Missing atoms46
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
MPHRIFGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRR
UniProt reference sequence
LIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLET
Aligned reference sequence
LIHG----------------EVLGKGC----------------FGQAIKVTH--RE------------------------------------TGEVMVMKELI--RFDEET----------------------------QRTFLKEVKVMRCL--------------------------EHPNVLKFIGVLYKDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLNFITEYIKG--------------GTLRGIIKS--MDS------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QYPWSQRVSFAKDIASGMAYLHSM---------------------------------------------NIIHRDL-----NSHNCLVRE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKNVVVADFGLARLMVDEKTQ--PEGLRSLKKPDRKKR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVVGNPYWMAPEMING--------------------------------RSYD-EKVDVFSFGIVLCEIIGR-------------------------VNADPDYLPRTM------------------------------------DFGLNVRGFLDRY--CPPNCP---------------------------------------------------------------------------------------------------------PSFFPITVRCCDLDPEKRPS--------------FVKLEHWLET
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
RIFG----------------EVLGKGC----------------FGQAIKVTH--RE------------------------------------TGEVMVMKELI--RFDEET----------------------------QRTFLKEVKVMRCL--------------------------EHPNVLKFIGVLY--K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLNFITEYIKG--------------GTLRGIIKS--MDS------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QYPWSQRVSFAKDIASGMAYLHSM---------------------------------------------NIIHRDL-----NSHNCLVRE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKNVVVADFGLARLM------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVVGNPYWMAPEMING--------------------------------RSYD-EKVDVFSFGIVLCEIIGR-------------------------VNADPDYLPRTM------------------------------------DFGLNVRGFLDRY--CPPNCP---------------------------------------------------------------------------------------------------------PSFFPITVRCCDLDPEKRPS--------------FVKLEHWLET
Activation segment
DFGLARLM------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVVGNPYWMAPE
Binding pocket
EVLGKGCFGQAIKMVMKELTFLKEVKVMRCLEPNVLKFIGVNFITEYIKGGTLRGIIKSYLHSMNIIHRDLNSHNCLVVADFGLA
Ligand info
Orthosteric ligand
RXN
Allosteric ligand
None
Ligand typeType3
Consensus conformation
DFG conformationout
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 7ATU, Chain A