6YTW Chain B
Dual specificity protein kinase CLK3 (CLK3)
Active — 98.8%DFG-inαC-inType1 · EAE
Resolution
2.0 Å
R-value
0.186
Predicted activity confidence98.8%
Structure info
Alternate conformationA
Missing atoms4
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
RSVEDDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCIAFELLGKNTFEFLKENNFQPYPLPHVRHMAYQLCHALRFLHENQLTHTDLKPENILFVNSEFETEKSVKNTSIRVADFGSATFDHEHHTTIVATRHYRPPEVILELGWAQPCDVWSIGCILFEYYRGFTLFQTHENREHLVMMEKILGPIPSHMIHRTRKQKYFYKGGLVWDENSSDGRYVKENCKPLKSYMLQDSLEHVQLFDLMRRMLEFDPAQRITLAEALLHPFFAGLTPEERS
UniProt reference sequence
YEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCIAFELLGKNTFEFLKENNFQPYPLPHVRHMAYQLCHALRFLHENQLTHTDLKPENILFVNSEFETLYNEHKSCEEKSVKNTSIRVADFGSATFDHEHHTTIVATRHYRPPEVILELGWAQPCDVWSIGCILFEYYRGFTLFQTHENREHLVMMEKILGPIPSHMIHRTRKQKYFYKGGLVWDENSSDGRYVKENCKPLKSYMLQDSLEHVQLFDLMRRMLEFDPAQRITLAEALLHPFF
Aligned reference sequence
YEIV----------------GNLGEGT----------------FGKVVECLD--HAR-----------------------------------GKSQVALKIIR--NVGKY-----------------------------REAARLEINVLKKI--KEKDKE------------------NKFLCVLMSDWFNFHG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HMCIAFELLG---------------KNTFEFLKE--NNFQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PYPLPHVRHMAYQLCHALRFLHEN---------------------------------------------QLTHTDL-----KPENILFVN--SEFETLYNEHKSCEEKSVK-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NTSIRVADFGSATFDHEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTIVATRHYRPPEVILE--------------------------------LGWA-QPCDVWSIGCILFEYYRG-----------------FTLFQT--------------------------------------------------HENREHLVMMEKI--LGPIPSHMIHRTRKQKYFYKGGLVWDENSSDGRYVKENCKPLKSYMLQDSLEH----------------------------------------------------------VQLFDLMRRMLEFDPAQRIT--------------LAEALLHPFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YEIV----------------GNLGEGT----------------FGKVVECLD--HAR-----------------------------------GKSQVALKIIR--NVGKY-----------------------------REAARLEINVLKKI--KEKDKE------------------NKFLCVLMSDWFNFHG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HMCIAFELLG---------------KNTFEFLKE--NNFQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PYPLPHVRHMAYQLCHALRFLHEN---------------------------------------------QLTHTDL-----KPENILFVN--SEFET---------EKSVK-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NTSIRVADFGSATFDHEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTIVATRHYRPPEVILE--------------------------------LGWA-QPCDVWSIGCILFEYYRG-----------------FTLFQT--------------------------------------------------HENREHLVMMEKI--LGPIPSHMIHRTRKQKYFYKGGLVWDENSSDGRYVKENCKPLKSYMLQDSLEH----------------------------------------------------------VQLFDLMRRMLEFDPAQRIT--------------LAEALLHPFF
Activation segment
DFGSATFDHEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTIVATRHYRPPE
Binding pocket
GNLGEGTFGKVVEVALKIIAARLEINVLKKIKFLCVLMSDWCIAFELLG_KNTFEFLKEFLHENQLTHTDLKPENILFVADFGSA
Ligand info
Orthosteric ligand
EAE
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6YTW, Chain B