6YTD Chain A
Dual specificity protein kinase CLK1 (CLK1)
Active — 99.5%DFG-inαC-inType1 · EAE
Resolution
2.0 Å
R-value
0.182
Predicted activity confidence99.5%
Structure info
Alternate conformationA
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
SMHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINPDIKVADFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRACKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRITLREALKHPFFDLLKKSI
UniProt reference sequence
YEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRRCKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRITLREALKHPFF
Aligned reference sequence
YEIV----------------DTLGEGA----------------FGKVVECID--HKA-----------------------------------GGRHVAVKIVK--NVDRY-----------------------------CEAARSEIQVLEHL--NTTDPN------------------STFRCVQMLEWFEHHG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HICIVFELLG---------------LSTYDFIKE--NGFL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFRLDHIRKMAYQICKSVNFLHSN---------------------------------------------KLTHTDL-----KPENILFVQ--SDYTEAYNPKIKRDERTLI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NPDIKVVDFGSATYDDEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STLVSTRHYRAPEVILA--------------------------------LGWS-QPCDVWSIGCILIEYYLG-----------------FTVFPT--------------------------------------------------HDSKEHLAMMERI--LGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRRCKPLKEFMLSQDVEH----------------------------------------------------------ERLFDLIQKMLEYDPAKRIT--------------LREALKHPFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YEIV----------------DTLGEGA----------------FGKVVECID--HKA-----------------------------------GGRHVAVKIVK--NVDRY-----------------------------CEAARSEIQVLEHL--NTTDPN------------------STFRCVQMLEWFEHHG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HICIVFELLG---------------LSTYDFIKE--NGFL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFRLDHIRKMAYQICKSVNFLHSN---------------------------------------------KLTHTDL-----KPENILFVQ--SDYTEAYNPKIKRDERTLI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NPDIKVADFGSATYDDEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STLVSTRHYRAPEVILA--------------------------------LGWS-QPCDVWSIGCILIEYYLG-----------------FTVFPT--------------------------------------------------HDSKEHLAMMERI--LGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRACKPLKEFMLSQDVEH----------------------------------------------------------ERLFDLIQKMLEYDPAKRIT--------------LREALKHPFF
Activation segment
DFGSATYDDEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STLVSTRHYRAPE
Binding pocket
DTLGEGAFGKVVEVAVKIVAARSEIQVLEHLNFRCVQMLEWCIVFELLG_LSTYDFIKEFLHSNKLTHTDLKPENILFVADFGSA
Ligand info
Orthosteric ligand
EAE
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6YTD, Chain A