6WPP Chain B
Mitogen-activated protein kinase kinase kinase 14 (MAP3K14)
Inactive — 38.0%DFG-inαC-inType1 · PZ4
Resolution
2.55 Å
R-value
0.207
Predicted activity confidence38.0%
Structure info
Alternate conformationA
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
GSVSSGQAHSLTSLAKTWAARDNEGVLLTEKLKPVDAEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVSLEVFRAEELMACAGLTSPRIVPLYGAVREGPAVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRH
UniProt reference sequence
WATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNR
Aligned reference sequence
WATH--Q-------------LRLGRGS----------------FGEVHRMED--KQ------------------------------------TGFQCAVKKVR--L---------------------------------EVFRAEELMACAGL--------------------------TSPRIVPLYGAVREGP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WVNIFMELLEG--------------GSLGQLVKE--QG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CLPEDRALYYLGQALEGLEYLHSR---------------------------------------------RILHGDV-----KADNVLLSS--D-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GSHAALCDFGHAVCLQPDGLG--KSLLTG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DYIPGTETHMAPEVVLG--------------------------------RSCD-AKVDVWSSCCMMLHMLNG-----------------CHPWTQ--------------------------------------------------FFRGPLCLKIASE--PPPVREIPPSCA---------------------------------------------------------------------------------------------------PLTAQAIQEGLRKEPIHRVS--------------AAELGGKVNR
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
WATH--Q-------------LRLGRGS----------------FGEVHRMED--KQ------------------------------------TGFQCAVKKVS--L---------------------------------EVFRAEELMACAGL--------------------------TSPRIVPLYGAVREGP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AVNIFMELLEG--------------GSLGQLVKE--QG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CLPEDRALYYLGQALEGLEYLHSR---------------------------------------------RILHGDV-----KADNVLLSS--D-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GSHAALCDFGHAVCLQP-------SLLTG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DYIPGTETHMAPEVVLG--------------------------------RSCD-AKVDVWSSCCMMLHMLNG-----------------CHPWTQ--------------------------------------------------FFRGPLCLKIASE--PPPVREIPPSCA---------------------------------------------------------------------------------------------------PLTAQAIQEGLRKEPIHRVS--------------AAELGGKVNR
Activation segment
DFGHAVCLQP-------SLLTG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DYIPGTETHMAPE
Binding pocket
LRLGRGSFGEVHRCAVKKVFRAEELMACAGLTPRIVPLYGANIFMELLEGGSLGQLVKEYLHSRRILHGDVKADNVLLLCDFGHA
Ligand info
Orthosteric ligand
PZ4
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6WPP, Chain B