6WJF Chain A
cAMP-dependent protein kinase catalytic subunit alpha (PRKACA)
Active — 99.6%DFG-inαC-out
Resolution
7.5 Å
R-value
999.0
Predicted activity confidence99.6%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
GKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKNKEPGAEEKFKEIAEAYDVLSDPRKREIFDRYGEEVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKGVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF
UniProt reference sequence
FERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWF
Aligned reference sequence
FERI----------------KTLGTGS----------------FGRVMLVKH--KE------------------------------------TGNHYAMKILD--KQKVVKLKQ-------------------------IEHTLNEKRILQAV--------------------------NFPFLVKLEFSFKDNS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLYMVMEYVPG--------------GEMFSHLRR--IG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RFSEPHARFYAAQIVLTFEYLHSL---------------------------------------------DLIYRDL-----KPENLLIDQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGYIQVTDFGFAKRVKGRT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WTLCGTPEYLAPEIILS--------------------------------KGYN-KAVDWWALGVLIYEMAAG-----------------YPPFFA--------------------------------------------------DQPIQIYEKIVSG--KVRFPSHFS------------------------------------------------------------------------------------------------------SDLKDLLRNLLQVDLTKRFG--NLKNG-------VNDIKNHKWF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FERI----------------KTLGTGS----------------FGRVMLVKH--KE------------------------------------TGNHYAMKILD--KQKVVKLKQ-------------------------IEHTLNEKRILQAV--------------------------NFPFLVKLEFSFKDNS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLYMVMEYVPG--------------GEMFSHLRR--IG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RFSEPHARFYAAQIVLTFEYLHSL---------------------------------------------DLIYRDL-----KPENLLIDQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGYIQVTDFGFAKRVKGRT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WTLCGTPEYLAPEIILS--------------------------------KGYN-KGVDWWALGVLIYEMAAG-----------------YPPFFA--------------------------------------------------DQPIQIYEKIVSG--KVRFPSHFS------------------------------------------------------------------------------------------------------SDLKDLLRNLLQVDLTKRFG--NLKNG-------VNDIKNHKWF
Activation segment
DFGFAKRVKGRT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WTLCGTPEYLAPE
Binding pocket
KTLGTGSFGRVMLYAMKILHTLNEKRILQAVNPFLVKLEFSYMVMEYVPGGEMFSHLRRYLHSLDLIYRDLKPENLLIVTDFGFA
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6WJF, Chain A