6S75 Chain D
Serine/threonine-protein kinase Nek7 (NEK7)
Inactive — 0.1%DFG-outαC-out
Resolution
3.3 Å
R-value
0.263
Predicted activity confidence0.1%
Structure info
Alternate conformation—
Missing atoms150
Missing residues3
Salt bridge (KinCore)Saltbr-none
Sequence info
PDB sequence
YNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACT
UniProt reference sequence
FRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKR
Aligned reference sequence
FRIE----------------KKIGRGQ----------------FSEVYRAAC--LL------------------------------------DGVPVALKKVQ--IFDLMDAKA-------------------------RADCIKEIDLLKQL--------------------------NHPNVIKYYASFIEDN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELNIVLELADA--------------GDLSRMIKH--FKKQKR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LIPERTVWKYFVQLCSALEHMHSR---------------------------------------------RVMHRDI-----KPANVFITA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGVVKLGDLGLGRFFSSKTTA--A---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HSLVGTPYYMSPERIHE--------------------------------NGYN-FKSDIWSLGCLLYEMAAL-----------------QSPFYG--DK----------------------------------------------MNLYSLCKKIEQC--DYPPLPSDHYS----------------------------------------------------------------------------------------------------EELRQLVNMCINPDPEKRPD--------------VTYVYDVAKR
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FRIE----------------KKIGRGQ----------------FSEVYRAAC--LL------------------------------------DGVPVALKKVQ--IFDLMDAKA-------------------------RADCIKEIDLLKQL--------------------------NHPNVIKYYASFIEDN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELNIVLELADA--------------GDLSRMIKH--FKKQKR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LIPERTVWKYFVQLCSALEHMHSR---------------------------------------------RVMHRDI-----KPANVFITA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGVVKLGDL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TPYYMSPERIHE--------------------------------NGYN-FKSDIWSLGCLLYEMAAL-----------------QSPFYG--DK----------------------------------------------MNLYSLCKKIEQC--DYPPLPSDHYS----------------------------------------------------------------------------------------------------EELRQLVNMCINPDPEKRPD--------------VTYVYDVAKR
Activation segment
DL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TPYYMSPE
Binding pocket
KKIGRGQFSEVYRVALKKVDCIKEIDLLKQLNPNVIKYYASNIVLELADAGDLSRMIKHHMHSRRVMHRDIKPANVFILGDL___
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationout
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6S75, Chain D