6Q8P Chain A
Dual specificity protein kinase CLK1 (CLK1)
Active — 91.4%DFG-inαC-inType1 · HQB
Resolution
3.0 Å
R-value
0.201
Predicted activity confidence91.4%
Structure info
Alternate conformation—
Missing atoms9
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
SMHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINPDIKVVDFGSATYDDEHHSTLVTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRACKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRITLREALKHPFFDLLKK
UniProt reference sequence
YEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRRCKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRITLREALKHPFF
Aligned reference sequence
YEIV----------------DTLGEGA----------------FGKVVECID--HKA-----------------------------------GGRHVAVKIVK--NVDRY-----------------------------CEAARSEIQVLEHL--NTTDPN------------------STFRCVQMLEWFEHHG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HICIVFELLG---------------LSTYDFIKE--NGFL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFRLDHIRKMAYQICKSVNFLHSN---------------------------------------------KLTHTDL-----KPENILFVQ--SDYTEAYNPKIKRDERTLI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NPDIKVVDFGSATYDDEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STLVSTRHYRAPEVILA--------------------------------LGWS-QPCDVWSIGCILIEYYLG-----------------FTVFPT--------------------------------------------------HDSKEHLAMMERI--LGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRRCKPLKEFMLSQDVEH----------------------------------------------------------ERLFDLIQKMLEYDPAKRIT--------------LREALKHPFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YEIV----------------DTLGEGA----------------FGKVVECID--HKA-----------------------------------GGRHVAVKIVK--NVDRY-----------------------------CEAARSEIQVLEHL--NTTDPN------------------STFRCVQMLEWFEHHG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HICIVFELLG---------------LSTYDFIKE--NGFL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFRLDHIRKMAYQICKSVNFLHSN---------------------------------------------KLTHTDL-----KPENILFVQ--SDYTEAYNPKIKRDERTLI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NPDIKVVDFGSATYDDEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STLV-TRHYRAPEVILA--------------------------------LGWS-QPCDVWSIGCILIEYYLG-----------------FTVFPT--------------------------------------------------HDSKEHLAMMERI--LGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRACKPLKEFMLSQDVEH----------------------------------------------------------ERLFDLIQKMLEYDPAKRIT--------------LREALKHPFF
Activation segment
DFGSATYDDEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STLV-TRHYRAPE
Binding pocket
DTLGEGAFGKVVEVAVKIVAARSEIQVLEHLNFRCVQMLEWCIVFELLG_LSTYDFIKEFLHSNKLTHTDLKPENILFVVDFGSA
Ligand info
Orthosteric ligand
HQB
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6Q8P, Chain A