6PJJ Chain A
Serine/threonine-protein kinase PRP4 homolog (PRPF4B)
Active — 98.6%DFG-inαC-inType1 · OCJ
Resolution
2.4 Å
R-value
0.193
Predicted activity confidence98.6%
Structure info
Alternate conformationA
Missing atoms12
Missing residues6
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
YYRVNIGEVLDKRYNVYGYTNVVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLLRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHVADNDITPLFSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLLDQILMLDPAKRISINQALQHAFIQE
UniProt reference sequence
YNVYGYTGQGVFSNVVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLFRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHVADNDITPYLVSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLLDQILMLDPAKRISINQALQHAFI
Aligned reference sequence
YNVY----------------GYTGQGV----------------FSNVVRARD--NAR-----------------------------------ANQEVAVKIIR--NNELM-----------------------------QKTGLKELEFLKKL--NDADPD------------------DKFHCLRLFRHFYHKQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HLCLVFEPLS---------------MNLREVLKK--YGKDV----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GLHIKAVRSYSQQLFLALKLLKRC---------------------------------------------NILHADI-----KPDNILVNE--S-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTILKLCDFGSASHVADNDI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TPYLVSRFYRAPEIIIG--------------------------------KSYD-YGIDMWSVGCTLYELYTG-----------------KILFPG--------------------------------------------------KTNNHMLKLAMDL--KGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKV------------------------------------------HQLKDLLDQILMLDPAKRIS--------------INQALQHAFI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YNVY----------------GYT----------------------NVVRARD--NAR-----------------------------------ANQEVAVKIIR--NNELM-----------------------------QKTGLKELEFLKKL--NDADPD------------------DKFHCLRLLRHFYHKQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HLCLVFEPLS---------------MNLREVLKK--YGKDV----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GLHIKAVRSYSQQLFLALKLLKRC---------------------------------------------NILHADI-----KPDNILVNE--S-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTILKLCDFGSASHVADNDI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TP-LFSRFYRAPEIIIG--------------------------------KSYD-YGIDMWSVGCTLYELYTG-----------------KILFPG--------------------------------------------------KTNNHMLKLAMDL--KGKMPNKMIRKGVFKDQHFDQNLNFMYIEV-----REKVTVMSTINPTKDLLADLIGCQRLPEDQRKKV------------------------------------------HQLKDLLDQILMLDPAKRIS--------------INQALQHAFI
Activation segment
DFGSASHVADNDI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TP-LFSRFYRAPE
Binding pocket
GYT______NVVRVAVKIITGLKELEFLKKLNFHCLRLLRHCLVFEPLS_MNLREVLKKLLKRCNILHADIKPDNILVLCDFGSA
Ligand info
Orthosteric ligand
OCJ
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6PJJ, Chain A