6N3L Chain A
eIF-2-alpha kinase GCN2 (EIF2AK4)
Inactive — 15.3%DFG-outαC-inType2 · KAV
Resolution
2.61 Å
R-value
0.226
Predicted activity confidence15.3%
Structure info
Alternate conformationA
Missing atoms0
Missing residues21
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
SRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIENVHYLYIQMEYCEASTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRNLKPVNIFLDSDDHVKIGDFAALYVSPEYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPFPEDFHAKQKSVISWLLNHDPAKRPTATELLKSELLP
UniProt reference sequence
VHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFI
Aligned reference sequence
VHKG----------------KCIGSDE--QL------------GKLVYNALE--TA------------------------------------TGGFVLLYEWV--LQWQKKMGPFLTSQEKEKIDKCKKQ---------IQGTETEFNSLVKL--------------------------SHPNVVRYLAMNLKEQ--DDSI--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VVDILVEHISG--------------VSLAAHLSH--SG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIPVHQLRRYTAQLLSGLDYLHSN---------------------------------------------SVVHKVL-----SASNVLVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGTVKITDYSISKRLADICKE------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVFEQTRVRFSDNALP---------------------------------YKTG-KKGDVWRLGLLLLSLSQG-------------------------QECG-----------------------------------------------------------EYPVTIPSDLP----------------------------------------------------------------------------------------------------ADFQDFLKKCVCLDDKERWS--------------PQQLLKHSFI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
SRYF----------------IEFEELQ--LL------------GKGAFGA----------------------------------------------------V--IKVQNKLDGCCYAVKRIPINPASRQ---------FRRIKGEVTLLSRL--------------------------HHENIVRY---YNAWI--ENVH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YLYIQMEYCEA--------------STLRDTIDQ--GL-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YRDTVRLWRLFREILDGLAYIHEK---------------------------------------------GMIHRNL-----KPVNIFLDS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DDHVKIGDFAALYVSPEYNKV------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLFSLGIIFFEMSYHP---------------------------------MVTA-SERIFVLNQL--------------------------------------------------------------------------------------------------RDPFPEDFH----------------------------------------------------------------------------------------------------AKQKSVISWLLNHDPAKRPT--------------ATELLKSELL
Activation segment
DFAALYVSPEYNKV------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLFSLGIIFFEMS
Binding pocket
QLLGKGAFGAVIKYAVKRIRIKGEVTLLSRLHENIVRYYNA__________________YIHEKGMIHRNLKPVNIFLIGDF___
Ligand info
Orthosteric ligand
KAV
Allosteric ligand
None
Ligand typeType2
Consensus conformation
DFG conformationout
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6N3L, Chain A