6LK5 Chain A
Mixed lineage kinase domain-like protein (MLKL)
Inactive — 1.5%DFG-inαC-in
Resolution
2.5 Å
R-value
0.196
Predicted activity confidence1.5%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
QIKEIKKEQLSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFS
UniProt reference sequence
LSGSPWILLRENEVSTLYKGEYHRAPVAIKVFKKLQAGSIAIVRQTFNKEIKTMKKFESPNILRIFGICIDETVTPPQFSIVMEYCELGTLRELLDREKDLTLGKRMVLVLGAARGLYRLHHSEAPELHGKIRSSNFLVTQGYQVKLAGFELRKTQTSMSLGTTREKTDRVKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLST
Aligned reference sequence
LSGS--PW------------ILLRENE----------------VSTLYKGEY----------------------------------------HRAPVAIKVFK--KLQAGSIAIV------------------------RQTFNKEIKTMKKF--------------------------ESPNILRIFGICIDET--VTPP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QFSIVMEYCEL--------------GTLRELLDR--EK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLTLGKRMVLVLGAARGLYRLHHS--EA-----------------------------------------PELHGKI-----RSSNFLVTQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GYQVKLAGFELRKTQTSMSLG--TTREK-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TDRVKSTAYLSPQELED--VF----------------------------YQYD-VKSEIYSFGIVLWEIATG-----------------DIPFQG--------------------------------------------------CNSEKIRKLVAVK--RQQEPLGEDCP----------------------------------------------------------------------------------------------------SELREIIDECRAHDPSVRPS--------------VDEILKKLST
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LSGS--PW------------ILLRENE----------------VSTLYKGEY----------------------------------------HRAPVAIKVFK--KLQAGSIAIV------------------------RQTFNKEIKTMKKF--------------------------ESPNILRIFGICIDET--VTPP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QFSIVMEYCEL--------------GTLRELLDR--EK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLTLGKRMVLVLGAARGLYRLHHS--EA-----------------------------------------PELHGKI-----RSSNFLVTQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GYQVKLAGFELRK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DRVKSTAYLSPQELED--VF----------------------------YQYD-VKSEIYSFGIVLWEIATG-----------------DIPFQG--------------------------------------------------CNSEKIRKLVAVK--RQQEPLGEDCP----------------------------------------------------------------------------------------------------SELREIIDECRAHDPSVRPS--------------VDEILKKLST
Activation segment
GFELRK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DRVKSTAYLSPQ
Binding pocket
ILLRENEVSTLYKVAIKVFTFNKEIKTMKKFEPNILRIFGISIVMEYCELGTLRELLDRHHSEAPELHGKIRSSNFLVLAGFELR
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6LK5, Chain A