6GES Chain A
Mitogen-activated protein kinase 3 (MAPK3)
Active — 94.9%DFG-inαC-inType1,Allosteric · 6H3
Resolution
2.07 Å
R-value
0.172
Predicted activity confidence94.9%
Structure info
Alternate conformationA
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
PGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPG
UniProt reference sequence
YTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYL
Aligned reference sequence
YTQL----------------QYIGEGA----------------YGMVSSAYD--HV------------------------------------RKTRVAIKKIS--PFEHQTY---------------------------CQRTLREIQILLRF--------------------------RHENVIGIRDILRAST--LEAMR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYIVQDLME---------------TDLYKLLKS--Q--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLSNDHICYFLYQILRGLKYIHSA---------------------------------------------NVLHRDL-----KPSNLLINT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCDLKICDFGLARIADPEHDH--TGFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TEYVATRWYRAPEIMLN--S-----------------------------KGYT-KSIDIWSVGCILAEMLSN-----------------RPIFPG--------------------------------------------------KHYLDQLNHILGI--LGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSD-----------------------------------------------------------------------SKALDLLDRMLTFNPNKRIT--------------VEEALAHPYL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YTQL----------------QYIGEGA----------------YGMVSSAYD--HV------------------------------------RKTRVAIKKIS--PFEHQTY---------------------------CQRTLREIQILLRF--------------------------RHENVIGIRDILRAST--LEAMR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYIVQDLME---------------TDLYKLLKS--Q--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLSNDHICYFLYQILRGLKYIHSA---------------------------------------------NVLHRDL-----KPSNLLINT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCDLKICDFGLARIADPEHDH--TGFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TEYVATRWYRAPEIMLN--S-----------------------------KGYT-KSIDIWSVGCILAEMLSN-----------------RPIFPG--------------------------------------------------KHYLDQLNHILGI--LGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSD-----------------------------------------------------------------------SKALDLLDRMLTFNPNKRIT--------------VEEALAHPYL
Activation segment
DFGLARIADPEHDH--TGFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TEYVATRWYRAPE
Binding pocket
QYIGEGAYGMVSSVAIKKIRTLREIQILLRFRENVIGIRDIYIVQDLME_TDLYKLLKSYIHSANVLHRDLKPSNLLIICDFGLA
Ligand info
Orthosteric ligand
6H3
Allosteric ligand
EWH
Ligand typeType1,Allosteric
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6GES, Chain A