6FYK Chain A
Dual specificity protein kinase CLK2 (CLK2)
Active — 99.9%DFG-inαC-inType1 · EAZ
Resolution
2.39 Å
R-value
0.196
Predicted activity confidence99.9%
Structure info
Alternate conformationA
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
SRRAKSVEDDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRKQKYFYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEEHHQLFDLIESMLEYEPAKRLTLGEALQHPFFARLRAE
UniProt reference sequence
YEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCISFELLGLSTFDFLKDNNYLPYPIHQVRHMAFQLCQAVKFLHDNKLTHTDLKPENILFVNSDYELTYNLEKKRDERSVKSTAVRVVDFGSATFDHEHHSTIVSTRHYRAPEVILELGWSQPCDVWSIGCIIFEYYVGFTLFQTHDNREHLAMMERILGPIPSRMIRKTRKQKYFYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEEHHQLFDLIESMLEYEPAKRLTLGEALQHPFF
Aligned reference sequence
YEIV----------------STLGEGT----------------FGRVVQCVD--HRR-----------------------------------GGARVALKIIK--NVEKY-----------------------------KEAARLEINVLEKI--NEKDPD------------------NKNLCVQMFDWFDYHG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HMCISFELLG---------------LSTFDFLKD--NNYL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PYPIHQVRHMAFQLCQAVKFLHDN---------------------------------------------KLTHTDL-----KPENILFVN--SDYELTYNLEKKRDERSVK-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STAVRVVDFGSATFDHEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STIVSTRHYRAPEVILE--------------------------------LGWS-QPCDVWSIGCIIFEYYVG-----------------FTLFQT--------------------------------------------------HDNREHLAMMERI--LGPIPSRMIRKTRKQKYFYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEEH----------------------------------------------------------HQLFDLIESMLEYEPAKRLT--------------LGEALQHPFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YEIV----------------STLGEGT----------------FGRVVQCVD--HRR-----------------------------------GGARVALKIIK--NVEKY-----------------------------KEAARLEINVLEKI--NEKDPD------------------NKNLCVQMFDWFDYHG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HMCISFELLG---------------LSTFDFLKD--NNYL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PYPIHQVRHMAFQLCQAVKFLHDN---------------------------------------------KLTHTDL-----KPENILFVN--SDYELTYNLEKKRDERSVK-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STAVRVVDFGSATFDHEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STIVSTRHYRAPEVILE--------------------------------LGWS-QPCDVWSIGCIIFEYYVG-----------------FTLFQT--------------------------------------------------HDNREHLAMMERI--LGPIPSRMIRKTRKQKYFYRGRLDWDENTSAGRYVRENCKPLRRYLTSEAEEH----------------------------------------------------------HQLFDLIESMLEYEPAKRLT--------------LGEALQHPFF
Activation segment
DFGSATFDHEHH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STIVSTRHYRAPE
Binding pocket
STLGEGTFGRVVQVALKIIAARLEINVLEKINNLCVQMFDWCISFELLG_LSTFDFLKDFLHDNKLTHTDLKPENILFVVDFGSA
Ligand info
Orthosteric ligand
EAZ
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6FYK, Chain A