6CCF Chain A
Calcium/calmodulin-dependent protein kinase kinase 1 (CAMKK1)
Active — 99.9%DFG-outαC-outType1 · H1N
Resolution
2.1 Å
R-value
0.212
Predicted activity confidence99.9%
Structure info
Alternate conformation—
Missing atoms31
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
MQLNQYKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEEPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTK
UniProt reference sequence
YKLQSEIGKGAYGVVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVNVVKLIEVLDDPAEDNLYLVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSNLLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGKCPFIDDFILALHRKIKNEPVVFPEEPEISEELKDLILKMLDKNPETRIGVPDIKLHPWV
Aligned reference sequence
YKLQ----------------SEIGKGA----------------YGVVRLAYN--ES------------------------------------EDRHYAMKVLS--KKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLP--LERVYQEIAILKKL--------------------------DHVNVVKLIEVLDDPA--ED----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLYLVFDLLRK--------------GPVMEVPCD--K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFSEEQARLYLRDVILGLEYLHCQ---------------------------------------------KIVHRDI-----KPSNLLLGD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGHVKIADFGVSNQFEGNDAQ--L---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SSTAGTPAFMAPEAISD--SG----------------------------QSFSGKALDVWATGVTLYCFVYG-----------------KCPFID--------------------------------------------------DFILALHRKIKNE--PVVFPEEPEIS----------------------------------------------------------------------------------------------------EELKDLILKMLDKNPETRIG--------------VPDIKLHPWV
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YKLQ----------------SEIGKGA----------------YGVVRLAYN--ES------------------------------------EDRHYAMKVLS--KKK-------------------------------LERVYQEIAILKKL--------------------------DHVNVVKLIEVLDDPA--ED----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLYLVFDLLRK--------------GPVMEVPCD--K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFSEEQARLYLRDVILGLEYLHCQ---------------------------------------------KIVHRDI-----KPSNLLLGD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGHVKIADFGVSNQFEGNDAQ--L---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SSTAGTPAFMAPEAISD--SG----------------------------QSFSGKALDVWATGVTLYCFVYG-----------------KCPFID--------------------------------------------------DFILALHRKIKNE--PVVFPEEPEIS----------------------------------------------------------------------------------------------------EELKDLILKMLDKNPETRIG--------------VPDIKLHPWV
Activation segment
DFGVSNQFEGNDAQ--L---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SSTAGTPAFMAPE
Binding pocket
QSEIGKGAYGVVRHYAMKVERVYQEIAILKKLHVNVVKLIENLYLVFDLRKGPVMEVPCEYLHCQKIVHRDIKPSNLLKIADFGV
Ligand info
Orthosteric ligand
H1N
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationout
αC-helix conformationout
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6CCF, Chain A