6C9G Chain A
5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1)
Active — 99.8%DFG-inαC-inAllosteric,Type1 · STU
Resolution
2.7 Å
R-value
0.205
Predicted activity confidence99.8%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
GSVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEIDNRRIMNEAKDFRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDELTPRPGSHTIEFFEMCANLIKILA
UniProt reference sequence
YILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWF
Aligned reference sequence
YILG----------------DTLGVGT----------------FGKVKVGKH--EL------------------------------------TGHKVAVKILN--RQKIRSLDV-------------------------VGKIRREIQNLKLF--------------------------RHPHIIKLYQVISTPS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DIFMVMEYVSG--------------GELFDYICK--NG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLDEKESRRLFQQILSGVDYCHRH---------------------------------------------MVVHRDL-----KPENVLLDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HMNAKIADFGLSNMMSDGEFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RTSCGSPNYAAPEVISG--------------------------------RLYAGPEVDIWSSGVILYALLCG-----------------TLPFDD--------------------------------------------------DHVPTLFKKICDG--IFYTPQYLN------------------------------------------------------------------------------------------------------PSVISLLKHMLQVDPMKRAT--------------IKDIREHEWF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YILG----------------DTLGVGT----------------FGKVKVGKH--EL------------------------------------TGHKVAVKILN--RQKIRSLDV-------------------------VGKIRREIQNLKLF--------------------------RHPHIIKLYQVISTPS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DIFMVMEYVSG--------------GELFDYICK--NG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLDEKESRRLFQQILSGVDYCHRH---------------------------------------------MVVHRDL-----KPENVLLDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HMNAKIADFGLSNMMSDGEFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R-SCGSPNYAAPEVISG--------------------------------RLYAGPEVDIWSSGVILYALLCG-----------------TLPFDD--------------------------------------------------DHVPTLFKKICDG--IFYTPQYLN------------------------------------------------------------------------------------------------------PSVISLLKHMLQVDPMKRAT--------------IKDIREHEWF
Activation segment
DFGLSNMMSDGEFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R-SCGSPNYAAPE
Binding pocket
DTLGVGTFGKVKVVAVKILKIRREIQNLKLFRPHIIKLYQVFMVMEYVSGGELFDYICKYCHRHMVVHRDLKPENVLLIADFGLS
Ligand info
Orthosteric ligand
STU
Allosteric ligand
R93
Ligand typeAllosteric,Type1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6C9G, Chain A