6C9D Chain B
Serine/threonine-protein kinase MARK1 (MARK1)
Active — 99.6%DFG-outαC-in
Resolution
2.5 Å
R-value
0.206
Predicted activity confidence99.6%
Structure info
Alternate conformationB
Missing atoms10
Missing residues7
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
EQPHIGNYRLQKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVGNKLDEFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWMNVGHEEEELKPYTEPDPDFNDTKRIDIMVTMGFARDEINDALINQKYDEVMATYILLGRKPPSKPRSLRFTWSMKTTSSMDPNDMMREIRKVLDANNCDYEQKERFLLFCVHGDARQDSLVQWEMEVCSLPSLSLNGVRFKRISGTSIAFKNIASKIANELKL
UniProt reference sequence
YRLQKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWM
Aligned reference sequence
YRLQ----------------KTIGKGN----------------FAKVKLARH--VL------------------------------------TGREVAVKIID--KTQLNPTS--------------------------LQKLFREVRIMKIL--------------------------NHPNIVKLFEVIETEK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLYLVMEYASG--------------GEVFDYLVA--HG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMKEKEARAKFRQIVSAVQYCHQK---------------------------------------------YIVHRDL-----KAENLLLDG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DMNIKIADFGFSNEFTVGNKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DTFCGSPPYAAPELFQG--------------------------------KKYDGPEVDVWSLGVILYTLVSG-----------------SLPFDG--------------------------------------------------QNLKELRERVLRG--KYRIPFYMS------------------------------------------------------------------------------------------------------TDCENLLKKLLVLNPIKRGS--------------LEQIMKDRWM
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YRLQ----------------KTIGKGN----------------FAKVKLARH--VL------------------------------------TGREVAVKIID--KTQLNPTS--------------------------LQKLFREVRIMKIL--------------------------NHPNIVKLFEVIETEK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLYLVMEYASG--------------GEVFDYLVA--HG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMKEKEARAKFRQIVSAVQYCHQK---------------------------------------------YIVHRDL-----KAENLLLDG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DMNIKIADFGFSNEFTVGNKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DEFCGSPPYAAPELFQG--------------------------------KKYDGPEVDVWSLGVILYTLVSG-----------------SLPFDG--------------------------------------------------QNLKELRERVLRG--KYRIPFYMS------------------------------------------------------------------------------------------------------TDCENLLKKLLVLNPIKRGS--------------LEQIMKDRWM
Activation segment
DFGFSNEFTVGNKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DEFCGSPPYAAPE
Binding pocket
KTIGKGNFAKVKLVAVKIIKLFREVRIMKILNPNIVKLFEVYLVMEYASGGEVFDYLVAYCHQKYIVHRDLKAENLLL_______
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationout
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6C9D, Chain B