6BRU Chain A
Serine/threonine-protein kinase VRK1 (VRK1)
Active — 100.0%DFG-inαC-in
Resolution
1.8 Å
R-value
0.196
Predicted activity confidence100.0%
Structure info
Alternate conformationB
Missing atoms4
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
AEQFAVGEIITDMAAAAWKVGLPIGQGGFGCIYLADMNSSESVGSDAPCVVKVEPSDNGPLFTELKFYQRAAKPEQIQKWIRTRKLKYLGVPKYWGSGLHDKNGKSYRFMIMDRFGSDLQKIYEANAKRFSRKTVLQLSLRILDILEYIHEHEYVHGDIKASNLLLNYKNPDQVYLVDYGLAYRYCPEGVHKAYAADPKRCHDGTIEFTSIDAHNGVAPSRRGDLEILGYCMIQWLTGHLPWEDNLKDPKYVRDSKIRYRENIASLMDKCFPAANAPGEIAKYMETVKLLDYTEKPLYENLRDILLQGLKAIGSKDDGKLDL
UniProt reference sequence
WKVGLPIGQGGFGCIYLADMNSSESVGSDAPCVVKVEPSDNGPLFTELKFYQRAAKPEQIQKWIRTRKLKYLGVPKYWGSGLHDKNGKSYRFMIMDRFGSDLQKIYEANAKRFSRKTVLQLSLRILDILEYIHEHEYVHGDIKASNLLLNYKNPDQVYLVDYGLAYRYCPEGVHKEYKEDPKRCHDGTIEFTSIDAHNGVAPSRRGDLEILGYCMIQWLTGHLPWEDNLKDPKYVRDSKIRYRENIASLMDKCFPEKNKPGEIAKYMETVKLLDYTEKPLYENLRDILLQ
Aligned reference sequence
WKVG----------------LPIGQGG----------------FGCIYLADM--NSSESVG-------------------------------SDAPCVVKVEP--SD--------------------------------NGPLFTELKFYQRA--AKPEQIQKWIRTRKLKY---------LGVPKYWGSGLHDK--NGKS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YRFMIMDRFG---------------SDLQKIYEA--NAK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RFSRKTVLQLSLRILDILEYIHEH---------------------------------------------EYVHGDI-----KASNLLLNY--KN----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PDQVYLVDYGLAYRYCPEGVH--KEYKEDPK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RCHDGTIEFTSIDAHNG--------------------------------VAPS-RRGDLEILGYCMIQWLTG-----------------HLPWED--NL----------------------------------------------KDPKYVRDSKIRY--RENIASLMDKCFPEKNKP---------------------------------------------------------------------------------------------GEIAKYMETVKLLDYTEKPL--------------YENLRDILLQ
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
WKVG----------------LPIGQGG----------------FGCIYLADM--NSSESVG-------------------------------SDAPCVVKVEP--SD--------------------------------NGPLFTELKFYQRA--AKPEQIQKWIRTRKLKY---------LGVPKYWGSGLHDK--NGKS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YRFMIMDRFG---------------SDLQKIYEA--NAK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RFSRKTVLQLSLRILDILEYIHEH---------------------------------------------EYVHGDI-----KASNLLLNY--KN----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PDQVYLVDYGLAYRYCPEGVH--KAYAADPK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RCHDGTIEFTSIDAHNG--------------------------------VAPS-RRGDLEILGYCMIQWLTG-----------------HLPWED--NL----------------------------------------------KDPKYVRDSKIRY--RENIASLMDKCFPAANAP---------------------------------------------------------------------------------------------GEIAKYMETVKLLDYTEKPL--------------YENLRDILLQ
Activation segment
DYGLAYRYCPEGVH--KAYAADPK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RCHDGTIEFTSID
Binding pocket
LPIGQGGFGCIYLCVVKVEPLFTELKFYQRAALGVPKYWGSFMIMDRFG_SDLQKIYEAYIHEHEYVHGDIKASNLLLLVDYGLA
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6BRU, Chain A