6AE3 Chain A
Glycogen synthase kinase-3 beta (GSK3B)
Active — 92.3%DFG-inαC-inType1 · MRI
Resolution
2.14 Å
R-value
0.192
Predicted activity confidence92.3%
Structure info
Alternate conformation—
Missing atoms14
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
KVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQRFKNRELQIMRKLDHCNIVRLRYFFYSSGKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAA
UniProt reference sequence
YTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFF
Aligned reference sequence
YTDT----------------KVIGNGS----------------FGVVYQAKL--CD------------------------------------SGELVAIKKVL--QD--------------------------------KRFKNRELQIMRKL--------------------------DHCNIVRLRYFFYSSG--EKKDEV------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YLNLVLDYVP---------------ETVYRVARH--YSRAKQ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLPVIYVKLYMYQLFRSLAYIHSF---------------------------------------------GICHRDI-----KPQNLLLDP--D-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAVLKLCDFGSAKQLVRGEPN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VSYICSRYYRAPELIFG--A-----------------------------TDYT-SSIDVWSAGCVLAELLLG-----------------QPIFPG--------------------------------------------------DSGVDQLVEIIKV--LGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTP-------------------------------------------------------------------------PEAIALCSRLLEYTPTARLT--------------PLEACAHSFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YTDT----------------KVIGNGS----------------FGVVYQAKL--CD------------------------------------SGELVAIKKVL--Q----------------------------------RFKNRELQIMRKL--------------------------DHCNIVRLRYFFYSSG---KKDEV------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YLNLVLDYVP---------------ETVYRVARH--YSRAKQ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLPVIYVKLYMYQLFRSLAYIHSF---------------------------------------------GICHRDI-----KPQNLLLDP--D-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TAVLKLCDFGSAKQLVRGEPN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VS-ICSRYYRAPELIFG--A-----------------------------TDYT-SSIDVWSAGCVLAELLLG-----------------QPIFPG--------------------------------------------------DSGVDQLVEIIKV--LGTPTREQIREMNPNYTE-KFPQIKAHPWTKVFRPRTP-------------------------------------------------------------------------PEAIALCSRLLEYTPTARLT--------------PLEACAHSFF
Activation segment
DFGSAKQLVRGEPN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VS-ICSRYYRAPE
Binding pocket
KVIGNGSFGVVYQVAIKKVFKNRELQIMRKLDCNIVRLRYFNLVLDYVP_ETVYRVARHYIHSFGICHRDIKPQNLLLLCDFGSA
Ligand info
Orthosteric ligand
MRI
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 6AE3, Chain A