5XVG Chain A
Serine/threonine-protein kinase PAK 4 (PAK4)
Active — 99.4%DFG-inαC-inType1 · 8FX
Resolution
2.1 Å
R-value
0.184
Predicted activity confidence99.4%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
SSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNRTR
UniProt reference sequence
LDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFL
Aligned reference sequence
LDNF----------------IKIGEGS----------------TGIVCIATV--RS------------------------------------SGKLVAVKKMD--LRKQQR----------------------------RELLFNEVVIMRDY--------------------------QHENVVEMYNSYLVGD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELWVVMEFLEG--------------GALTDIVTH--T--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMNEEQIAAVCLAVLQALSVLHAQ---------------------------------------------GVIHRDI-----KSDSILLTH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGRVKLSDFGFCAQVSKEVPR--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KSLVGTPYWMAPELISR--------------------------------LPYG-PEVDIWSLGIMVIEMVDG-----------------EPPYFN--------------------------------------------------EPPLKAMKMIRDN--LPPRLKNLHKVS---------------------------------------------------------------------------------------------------PSLKGFLDRLLVRDPAQRAT--------------AAELLKHPFL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LDNF----------------IKIGEGS----------------TGIVCIATV--RS------------------------------------SGKLVAVKKMD--LRKQQR----------------------------RELLFNEVVIMRDY--------------------------QHENVVEMYNSYLVGD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELWVVMEFLEG--------------GALTDIVTH--T--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMNEEQIAAVCLAVLQALSVLHAQ---------------------------------------------GVIHRDI-----KSDSILLTH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGRVKLSDFGFCAQVSKEVPR--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K-LVGTPYWMAPELISR--------------------------------LPYG-PEVDIWSLGIMVIEMVDG-----------------EPPYFN--------------------------------------------------EPPLKAMKMIRDN--LPPRLKNLHKVS---------------------------------------------------------------------------------------------------PSLKGFLDRLLVRDPAQRAT--------------AAELLKHPFL
Activation segment
DFGFCAQVSKEVPR--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K-LVGTPYWMAPE
Binding pocket
IKIGEGSTGIVCIVAVKKMLLFNEVVIMRDYQENVVEMYNSWVVMEFLEGGALTDIVTHVLHAQGVIHRDIKSDSILLLSDFGFC
Ligand info
Orthosteric ligand
8FX
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5XVG, Chain A