5XS2 Chain A
Cyclin-dependent kinase 8 (CDK8)
Active — 98.5%DFG-inαC-inType1 · 8D6
Resolution
2.04 Å
R-value
0.197
Predicted activity confidence98.5%
Structure info
Alternate conformationB
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
MEFMDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQIEGTGISMSACREIALLRELKHPNVISLQKVFLSHADRKVWLLFDYAEHDLWHIIKFHRASKANKKPVQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPADKDWEDIKKMPEHSTLMKDFRRNTYTNCSLIKYMEKHKVKPDSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLEDPLPTSDVFAGCQIPYPKREFLTEEE
UniProt reference sequence
FEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQIEGTGISMSACREIALLRELKHPNVISLQKVFLSHADRKVWLLFDYAEHDLWHIIKFHRASKANKKPVQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPADKDWEDIKKMPEHSTLMKDFRRNTYTNCSLIKYMEKHKVKPDSKAFHLLQKLLTMDPIKRITSEQAMQDPYF
Aligned reference sequence
FEYE--G-------------CKVGRGT----------------YGHVYKAKR--KDGK----------------------------------DDKDYALKQIE--GTGI------------------------------SMSACREIALLREL--------------------------KHPNVISLQKVFLSHA--DR----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KVWLLFDYAE---------------HDLWHIIKF--HRASKANKKPV----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLPRGMVKSLLYQILDGIHYLHAN---------------------------------------------WVLHRDL-----KPANILVMG--EGPE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGRVKIADMGFARLFNSPLKP--LADL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DPVVVTFWYRAPELLLG--A-----------------------------RHYT-KAIDIWAIGCIFAELLTS-----------------EPIFHC--RQEDIKTSN---------------------------------------PYHHDQLDRIFNV--MGFPADKDWEDIKKMPEHSTLMKDFRRNTYTNCSLIKYMEKHKVKPD----------------------------------------------------------------SKAFHLLQKLLTMDPIKRIT--------------SEQAMQDPYF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FEYE--G-------------CKVGRGT----------------YGHVYKAKR--KDGK----------------------------------DDKDYALKQIE--GTGI------------------------------SMSACREIALLREL--------------------------KHPNVISLQKVFLSHA--DR----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KVWLLFDYAE---------------HDLWHIIKF--HRASKANKKPV----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLPRGMVKSLLYQILDGIHYLHAN---------------------------------------------WVLHRDL-----KPANILVMG--EGPE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGRVKIADMGFARLFNSPLKP--LADL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DPVVVTFWYRAPELLLG--A-----------------------------RHYT-KAIDIWAIGCIFAELLTS-----------------EPIFHC--RQEDIKTSN---------------------------------------PYHHDQLDRIFNV--MGFPADKDWEDIKKMPEHSTLMKDFRRNTYTNCSLIKYMEKHKVKPD----------------------------------------------------------------SKAFHLLQKLLTMDPIKRIT--------------SEQAMQDPYF
Activation segment
DMGFARLFNSPLKP--LADL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DPVVVTFWYRAPE
Binding pocket
CKVGRGTYGHVYKYALKQISACREIALLRELKPNVISLQKVWLLFDYAE_HDLWHIIKFYLHANWVLHRDLKPANILVIADMGFA
Ligand info
Orthosteric ligand
8D6
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5XS2, Chain A