5WE8 Chain A
Serine/threonine-protein kinase WNK1 (WNK1)
Active — 100.0%DFG-inαC-outATPlike,Type3 · ANP
Resolution
2.01 Å
R-value
0.205
Predicted activity confidence100.0%
Structure info
Alternate conformationB
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-na
Sequence info
PDB sequence
TKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRADFAKSVIGTPEFMAPEMYAAAYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEET
UniProt reference sequence
FLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFF
Aligned reference sequence
FLKF--D-------------IEIGRGS----------------FKTVYKGLD--TE------------------------------------TTVEVAWCELQ--DRKLTKSE--------------------------RQRFKEEAEMLKGL--------------------------QHPNIVRFYDSWESTV--KGKK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CIVLVTELMTS--------------GTLKTYLKR--FK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VMKIKVLRSWCRQILKGLQFLHTR--TP-----------------------------------------PIIHRDL-----KCDNIFITG--P-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGSVKIGDLGLATLKRASFA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KSVIGTPEFMAPEMYE---------------------------------EKYD-ESVDVYAFGMCMLEMATS-----------------EYPYSE--C-----------------------------------------------QNAAQIYRRVTSG--VKPASFDKVAI----------------------------------------------------------------------------------------------------PEVKEIIEGCIRQNKDERYS--------------IKDLLNHAFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FLKF--D-------------IEIGRGS----------------FKTVYKGLD--TE------------------------------------TTVEVAWCELQ--DRKLTKSE--------------------------RQRFKEEAEMLKGL--------------------------QHPNIVRFYDSWEST---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CIVLVTELMTS--------------GTLKTYLKR--FK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VMKIKVLRSWCRQILKGLQFLHTR--TP-----------------------------------------PIIHRDL-----KCDNIFITG--P-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGSVKIGDLGLATLKRADFA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KSVIGTPEFMAPEMYA---------------------------------AAYD-ESVDVYAFGMCMLEMATS-----------------EYPYSE--C-----------------------------------------------QNAAQIYRRVTSG--VKPASFDKVAI----------------------------------------------------------------------------------------------------PEVKEIIEGCIRQNKDERYS--------------IKDLLNHAFF
Activation segment
DLGLATLKRADFA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KSVIGTPEFMAPE
Binding pocket
IEIGRGSFKTVYKVAWCELRFKEEAEMLKGLQPNIVRFYDSVLVTELMTSGTLKTYLKRHTRTPPIIHRDLKCDNIFIIGDLGLA
Ligand info
Orthosteric ligand
ANP
Allosteric ligand
None
Ligand typeATPlike,Type3
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5WE8, Chain A