5VO2 Chain A
Mitogen-activated protein kinase kinase kinase 12 (MAP3K12)
Inactive — 0.0%DFG-inαC-outType1 · 9FV
Resolution
2.96 Å
R-value
0.174
Predicted activity confidence0.0%
Structure info
Alternate conformationA
Missing atoms0
Missing residues2
Salt bridge (KinCore)Saltbr-na
Sequence info
PDB sequence
LWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWREEVKLHFEKIK
UniProt reference sequence
ILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFKGVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYDDVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSAIIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDI
Aligned reference sequence
ILDL----------------QWVGSGA----------------QGAVFLGRF----------------------------------------HGEEVAVKKVR-------------------------------------DLKETDIKHLRKL--------------------------KHPNIITFKGVCTQAP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CYCILMEFCAQ--------------GQLYEVLRA--GR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PVTPSLLVDWSMGIAGGMNYLHLH---------------------------------------------KIIHRDL-----KSPNMLITY--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DDVVKISDFGTSKELSDKSTK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MSFAGTVAWMAPEVIRN--------------------------------EPVS-EKVDIWSFGVVLWELLTG-----------------EIPYKD--------------------------------------------------VDSSAIIWGVGSN--SLHLPVPSSCP----------------------------------------------------------------------------------------------------DGFKILLRQCWNSKPRNRPS--------------FRQILLHLDI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
ILDL----------------QWVGSGA----------------QGAVFLGRF----------------------------------------HGEEVAVKKVR-------------------------------------DLKETDIKHLRKL--------------------------KHPNIITFKGVCTQAP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CYCILMEFCAQ--------------GQLYEVLRA--GR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PVTPSLLVDWSMGIAGGMNYLHLH---------------------------------------------KIIHRDL-----KSPNMLITY--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DDVVKISDFG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FAGTVAWMAPEVIRN--------------------------------EPVS-EKVDIWSFGVVLWELLTG-----------------EIPYKD--------------------------------------------------VDSSAIIWGVGSN--SLHLPVPSSCP----------------------------------------------------------------------------------------------------DGFKILLRQCWNSKPRNRPS--------------FRQILLHLDI
Activation segment
DFG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FAGTVAWMAPE
Binding pocket
QWVGSGAQGAVFLVAVKKVLKETDIKHLRKLKPNIITFKGVCILMEFCAQGQLYEVLRAYLHLHKIIHRDLKSPNMLIISDFG__
Ligand info
Orthosteric ligand
9FV
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5VO2, Chain A