5VED Chain A
Serine/threonine-protein kinase PAK 4 (PAK4)
Active — 100.0%DFG-inαC-inType1 · STU
Resolution
2.3 Å
R-value
0.231
Predicted activity confidence100.0%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
SHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNR
UniProt reference sequence
LDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFL
Aligned reference sequence
LDNF----------------IKIGEGS----------------TGIVCIATV--RS------------------------------------SGKLVAVKKMD--LRKQQR----------------------------RELLFNEVVIMRDY--------------------------QHENVVEMYNSYLVGD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELWVVMEFLEG--------------GALTDIVTH--T--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMNEEQIAAVCLAVLQALSVLHAQ---------------------------------------------GVIHRDI-----KSDSILLTH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGRVKLSDFGFCAQVSKEVPR--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KSLVGTPYWMAPELISR--------------------------------LPYG-PEVDIWSLGIMVIEMVDG-----------------EPPYFN--------------------------------------------------EPPLKAMKMIRDN--LPPRLKNLHKVS---------------------------------------------------------------------------------------------------PSLKGFLDRLLVRDPAQRAT--------------AAELLKHPFL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LDNF----------------IKIGEGS----------------TGIVCIATV--RS------------------------------------SGKLVAVKKMD--LRKQQR----------------------------RELLFNEVVIMRDY--------------------------QHENVVEMYNSYLVGD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELWVVMEFLEG--------------GALTDIVTH--T--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMNEEQIAAVCLAVLQALSVLHAQ---------------------------------------------GVIHRDI-----KSDSILLTH--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGRVKLSDFGFCAQVSKEVPR--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K-LVGTPYWMAPELISR--------------------------------LPYG-PEVDIWSLGIMVIEMVDG-----------------EPPYFN--------------------------------------------------EPPLKAMKMIRDN--LPPRLKNLHKVS---------------------------------------------------------------------------------------------------PSLKGFLDRLLVRDPAQRAT--------------AAELLKHPFL
Activation segment
DFGFCAQVSKEVPR--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K-LVGTPYWMAPE
Binding pocket
IKIGEGSTGIVCIVAVKKMLLFNEVVIMRDYQENVVEMYNSWVVMEFLEGGALTDIVTHVLHAQGVIHRDIKSDSILLLSDFGFC
Ligand info
Orthosteric ligand
STU
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5VED, Chain A