5UVF Chain C
Serine/threonine-protein kinase VRK1 (VRK1)
Active — 98.2%DFG-inαC-inType1.5_Back · 7DZ
Resolution
2.0 Å
R-value
0.175
Predicted activity confidence98.2%
Structure info
Alternate conformation—
Missing atoms20
Missing residues4
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
FAVGEIITDMAAAAWKVGLPIGQCIYLADMNDAPCVVKVEPSGPLFTELKFYQRAAKPEQIQKWIRTRKLKYLGVPKYWGSGLHSYRFMIMDRFGSDLQKIYEANAKRFSRKTVLQLSLRILDILEYIHEHEYVHGDIKASNLLLNYKNPDQVYLVDYGLAYRYCPEGVHKAYAADPKRCHDGTIEFTSIDAHNGVAPSRRGDLEILGYCMIQWLTGHLPWEDNLKDPKYVRDSKIRYRENIASLMDKCFPAANAPGEIAKYMETVKLLDYTEKPLYENLRDILLQGLKAIGSKDDGKLDL
UniProt reference sequence
WKVGLPIGQGGFGCIYLADMNSSESVGSDAPCVVKVEPSDNGPLFTELKFYQRAAKPEQIQKWIRTRKLKYLGVPKYWGSGLHDKNGKSYRFMIMDRFGSDLQKIYEANAKRFSRKTVLQLSLRILDILEYIHEHEYVHGDIKASNLLLNYKNPDQVYLVDYGLAYRYCPEGVHKEYKEDPKRCHDGTIEFTSIDAHNGVAPSRRGDLEILGYCMIQWLTGHLPWEDNLKDPKYVRDSKIRYRENIASLMDKCFPEKNKPGEIAKYMETVKLLDYTEKPLYENLRDILLQ
Aligned reference sequence
WKVG----------------LPIGQGG----------------FGCIYLADM--NSSESVG-------------------------------SDAPCVVKVEP--SD--------------------------------NGPLFTELKFYQRA--AKPEQIQKWIRTRKLKY---------LGVPKYWGSGLHDK--NGKS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YRFMIMDRFG---------------SDLQKIYEA--NAK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RFSRKTVLQLSLRILDILEYIHEH---------------------------------------------EYVHGDI-----KASNLLLNY--KN----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PDQVYLVDYGLAYRYCPEGVH--KEYKEDPK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RCHDGTIEFTSIDAHNG--------------------------------VAPS-RRGDLEILGYCMIQWLTG-----------------HLPWED--NL----------------------------------------------KDPKYVRDSKIRY--RENIASLMDKCFPEKNKP---------------------------------------------------------------------------------------------GEIAKYMETVKLLDYTEKPL--------------YENLRDILLQ
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
WKVG----------------LPIGQ--------------------CIYLADM--N--------------------------------------DAPCVVKVEP--S----------------------------------GPLFTELKFYQRA--AKPEQIQKWIRTRKLKY---------LGVPKYWGSGLH-------S--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YRFMIMDRFG---------------SDLQKIYEA--NAK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RFSRKTVLQLSLRILDILEYIHEH---------------------------------------------EYVHGDI-----KASNLLLNY--KN----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PDQVYLVDYGLAYRYCPEGVH--KAYAADPK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RCHDGTIEFTSIDAHNG--------------------------------VAPS-RRGDLEILGYCMIQWLTG-----------------HLPWED--NL----------------------------------------------KDPKYVRDSKIRY--RENIASLMDKCFPAANAP---------------------------------------------------------------------------------------------GEIAKYMETVKLLDYTEKPL--------------YENLRDILLQ
Activation segment
DYGLAYRYCPEGVH--KAYAADPK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RCHDGTIEFTSID
Binding pocket
LPIGQ____CIYLCVVKVEPLFTELKFYQRAALGVPKYWGSFMIMDRFG_SDLQKIYEAYIHEHEYVHGDIKASNLLLLVDYGLA
Ligand info
Orthosteric ligand
7DZ
Allosteric ligand
None
Ligand typeType1.5_Back
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5UVF, Chain C