5MZL Chain A
Serine/threonine-protein kinase pim-1 (PIM1)
Active — 99.8%DFG-inαC-inType1 · 8ET
Resolution
1.96 Å
R-value
0.158
Predicted activity confidence99.8%
Structure info
Alternate conformationB
Missing atoms8
Missing residues4
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
PLESQYQVGPLLGSSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIGGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLH
UniProt reference sequence
YQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWM
Aligned reference sequence
YQVG----------------PLLGSGG----------------FGSVYSGIR--VS------------------------------------DNLPVAIKHVE--KDRISDWGELPN----------------------GTRVPMEVVLLKKV--SS----------------------GFSGVIRLLDWFERPD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SFVLILERPEP--V-----------QDLFDFITE--RG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALQEELARSFFWQVLEAVRHCHNC---------------------------------------------GVLHRDI-----KDENILIDL--N-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGELKLIDFGSGALLKDTVY-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TDFDGTRVYSPPEWIRY--------------------------------HRYHGRSAAVWSLGILLYDMVCG-----------------DIPFE--------------------------------------------------------HDEEIIRG--QVFFRQRVS------------------------------------------------------------------------------------------------------SECQHLIRWCLALRPSDRPT--------------FEEIQNHPWM
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YQVG----------------PLLGS--------------------SVYSGIR--VS------------------------------------DNLPVAIKHVE--KDRISDWGELPN----------------------GTRVPMEVVLLKKV--SS----------------------GFSGVIRLLDWFERPD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SFVLILERPEP--V-----------QDLFDFITE--RG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALQEELARSFFWQVLEAVRHCHNC---------------------------------------------GVLHRDI-----KDENILIDL--N-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGELKLIDFGSGALLKDTVY-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TDFDGTRVYSPPEWIRY--------------------------------HRYHGRSAAVWSLGILLYDMVCG-----------------DIPFE--------------------------------------------------------HDEEIIGG--QVFFRQRVS------------------------------------------------------------------------------------------------------SECQHLIRWCLALRPSDRPT--------------FEEIQNHPWM
Activation segment
DFGSGALLKDTVY-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TDFDGTRVYSPPE
Binding pocket
PLLGS____SVYSVAIKHVRVPMEVVLLKKVSSGVIRLLDWVLILERPEVQDLFDFITEHCHNCGVLHRDIKDENILILIDFGSG
Ligand info
Orthosteric ligand
8ET
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5MZL, Chain A