5L4Q Chain B
AP2-associated protein kinase 1 (AAK1)
Active — 98.6%DFG-inαC-inType1 · LKB
Resolution
1.97 Å
R-value
0.208
Predicted activity confidence98.6%
Structure info
Alternate conformationB
Missing atoms7
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
GYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKL
UniProt reference sequence
VTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFK
Aligned reference sequence
VTVD----------------EVLAEGG----------------FAIVFLVRT--S-------------------------------------NGMKCALKRMF--VNNEHD----------------------------LQVCKREIQIMRDL--S-----------------------GHKNIVGYIDSSINNV--SSGDVW------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EVLILMDFCRG--------------GQVVNLMNQ--RLQT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GFTENEVLQIFCDTCEAVARLHQC--KT-----------------------------------------PIIHRDL-----KVENILLHD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGHYVLCDFGSATNKFQNPQT--EGVNAVEDE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IKKYTTLSYRAPEMVNL--YSG---------------------------KIIT-TKADIWALGCLLYKLCYF-----------------TLPFGE-------------------------------------------------------SQVAICDG--NFTIPDNSRYS----------------------------------------------------------------------------------------------------QDMHCLIRYMLEPDPDKRPD--------------IYQVSYFSFK
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
VTVD----------------EVLAEGG----------------FAIVFLVRT--S-------------------------------------NGMKCALKRMF--VNNEHD----------------------------LQVCKREIQIMRDL--S-----------------------GHKNIVGYIDSSINNV------VW------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EVLILMDFCRG--------------GQVVNLMNQ--RLQT-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GFTENEVLQIFCDTCEAVARLHQC--KT-----------------------------------------PIIHRDL-----KVENILLHD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGHYVLCDFGSATNKFQNPQT--EGVNAVEDE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IKKYTTLSYRAPEMVNL--YSG---------------------------KIIT-TKADIWALGCLLYKLCYF-----------------TLPF-------------------------------------------------------------------NFTIPDNSRYS----------------------------------------------------------------------------------------------------QDMHCLIRYMLEPDPDKRPD--------------IYQVSYFSFK
Activation segment
DFGSATNKFQNPQT--EGVNAVEDE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IKKYTTLSYRAPE
Binding pocket
EVLAEGGFAIVFLCALKRMVCKREIQIMRDLSKNIVGYIDSLILMDFCRGGQVVNLMNQHQCKTPIIHRDLKVENILLLCDFGSA
Ligand info
Orthosteric ligand
LKB
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5L4Q, Chain B