5KZ8 Chain A
Serine/threonine-protein kinase MARK2 (MARK2)
Inactive — 0.2%DFG-inαC-inType1 · 6Z5
Resolution
3.21 Å
R-value
0.222
Predicted activity confidence0.2%
Structure info
Alternate conformation—
Missing atoms7
Missing residues2
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
PHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMATYLLLGY
UniProt reference sequence
YRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWM
Aligned reference sequence
YRLL----------------KTIGKGN----------------FAKVKLARH--IL------------------------------------TGKEVAVKIID--KTQLNSSS--------------------------LQKLFREVRIMKVL--------------------------NHPNIVKLFEVIETEK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLYLVMEYASG--------------GEVFDYLVA--HG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMKEKEARAKFRQIVSAVQYCHQK---------------------------------------------FIVHRDL-----KAENLLLDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DMNIKIADFGFSNEFTFGNKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DTFCGSPPYAAPELFQG--------------------------------KKYDGPEVDVWSLGVILYTLVSG-----------------SLPFDG--------------------------------------------------QNLKELRERVLRG--KYRIPFYMS------------------------------------------------------------------------------------------------------TDCENLLKKFLILNPSKRGT--------------LEQIMKDRWM
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YRLL----------------KTIGKGN----------------FAKVKLARH--IL------------------------------------TGKEVAVKIID--KTQLNSSS--------------------------LQKLFREVRIMKVL--------------------------NHPNIVKLFEVIETEK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLYLVMEYASG--------------GEVFDYLVA--HG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMKEKEARAKFRQIVSAVQYCHQK---------------------------------------------FIVHRDL-----KAENLLLDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DMNIKIADFG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGSPPYAAPELFQG--------------------------------KKYDGPEVDVWSLGVILYTLVSG-----------------SLPFDG--------------------------------------------------QNLKELRERVLRG--KYRIPFYMS------------------------------------------------------------------------------------------------------TDCENLLKKFLILNPSKRGT--------------LEQIMKDRWM
Activation segment
DFG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CGSPPYAAPE
Binding pocket
KTIGKGNFAKVKLVAVKIIKLFREVRIMKVLNPNIVKLFEVYLVMEYASGGEVFDYLVAYCHQKFIVHRDLKAENLLLIADFG__
Ligand info
Orthosteric ligand
6Z5
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5KZ8, Chain A