5KCV Chain A
RAC-alpha serine/threonine-protein kinase (AKT1)
Inactive — 0.0%DFG-outαC-outAllosteric · 6S1
Resolution
2.7 Å
R-value
0.213
Predicted activity confidence0.0%
Structure info
Alternate conformation—
Missing atoms37
Missing residues7
Salt bridge (KinCore)Saltbr-none
Sequence info
PDB sequence
IVKEGWLHKRGEYITWRPRYFLLKNDGTFIGYKERPAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYF
UniProt reference sequence
FEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFF
Aligned reference sequence
FEYL----------------KLLGKGT----------------FGKVILVKE--KA------------------------------------TGRYYAMKILK--KEVIVAKDE-------------------------VAHTLTENRVLQNS--------------------------RHPFLTALKYSFQTHD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLCFVMEYANG--------------GELFFHLSR--ER-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VFSEDRARFYGAEIVSALDYLHSE--K------------------------------------------NVVYRDL-----KLENLMLDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGHIKITDFGLCKEGIKDGAT--M---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTFCGTPEYLAPEVLED--------------------------------NDYG-RAVDWWGLGVVMYEMMCG-----------------RLPFYN--------------------------------------------------QDHEKLFELILME--EIRFPRTLG------------------------------------------------------------------------------------------------------PEAKSLLSGLLKKDPKQRLG--GGSED-------AKEIMQHRFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FEYL----------------KLLGKGT----------------FGKVILVKE--KA------------------------------------TGRYYAMKILK--K------------------------------------------VLQNS--------------------------RHPFLTALKYSFQTHD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLCFVMEYANG--------------GELFFHLSR--ER-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VFSEDRARFYGAEIVSALDYLHSE--K------------------------------------------NVVYRDL-----KLENLMLDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGHIKITDFGLC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PEYLAPEVLED--------------------------------NDYG-RAVDWWGLGVVMYEMMCG-----------------RLPFYN--------------------------------------------------QDHEKLFELILME--EIRFPRTLG------------------------------------------------------------------------------------------------------PEAKSLLSGLLKKDPKQRLG--GGSED-------AKEIMQHRFF
Activation segment
DFGLC---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PEYLAPE
Binding pocket
KLLGKGTFGKVILYAMKIL_______VLQNSRPFLTALKYSCFVMEYANGGELFFHLSRLHSEKNVVYRDLKLENLMLITDFGLC
Ligand info
Orthosteric ligand
6S1
Allosteric ligand
None
Ligand typeAllosteric
Consensus conformation
DFG conformationout
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5KCV, Chain A