5JRQ Chain A
Serine/threonine-protein kinase B-raf (BRAF)
Inactive — 5.2%DFG-inαC-outType1.5_Back · 6N9
Resolution
2.29 Å
R-value
0.2
Predicted activity confidence5.2%
Structure info
Alternate conformation—
Missing atoms16
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
FDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHASETKFEMKKLIDIARQTARGMDYLHAKSIIHRDLKSNNIFLHEDNTVKIGDFGLATEKSRSILWMAPEVIRMNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIEMVGRGSLSPDLSKVRSNCPKRMKRLMAECLKKKRDERPSFPRILAEIEELAR
UniProt reference sequence
ITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIEL
Aligned reference sequence
ITVG----------------QRIGSGS----------------FGTVYKGKW-----------------------------------------HGDVAVKMLN--VTAPTPQQ--------------------------LQAFKNEVGVLRKT--------------------------RHVNILLFMGYSTKP---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLAIVTQWCEG--------------SSLYHHLHI--IET------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KFEMIKLIDIARQTAQGMDYLHAK---------------------------------------------SIIHRDL-----KSNNIFLHE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLTVKIGDFGLATVKSRWSGS--HQF-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EQLSGSILWMAPEVIRM--QDK---------------------------NPYS-FQSDVYAFGIVLYELMTG-----------------QLPYSN--I-----------------------------------------------NNRDQIIFMVGRG--YLSPDLSKVRSNCP-------------------------------------------------------------------------------------------------KAMKRLMAECLKKKRDERPL--------------FPQILASIEL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
ITVG----------------QRIGSGS----------------FGTVYKGKW-----------------------------------------HGDVAVKMLN--VTAPTPQQ--------------------------LQAFKNEVGVLRKT--------------------------RHVNILLFMGYSTKP---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLAIVTQWCEG--------------SSLYHHLHA--SET------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KFEMKKLIDIARQTARGMDYLHAK---------------------------------------------SIIHRDL-----KSNNIFLHE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DNTVKIGDFGLATEKSR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SILWMAPEVIRM--------------------------------NPYS-FQSDVYAFGIVLYELMTG-----------------QLPYSN--I-----------------------------------------------NNRDQIIEMVGRG--SLSPDLSKVRSNCP-------------------------------------------------------------------------------------------------KRMKRLMAECLKKKRDERPS--------------FPRILAEIER
Activation segment
DFGLATEKSR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SILWMAPE
Binding pocket
QRIGSGSFGTVYKVAVKMLAFKNEVGVLRKTRVNILLFMGYAIVTQWCEGSSLYHHLHAYLHAKSIIHRDLKSNNIFLIGDFGLA
Ligand info
Orthosteric ligand
6N9
Allosteric ligand
None
Ligand typeType1.5_Back
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5JRQ, Chain A