5J0A Chain A
Lymphokine-activated killer T-cell-originated protein kinase (PBK)
Inactive — 0.7%DFG-inαC-out
Resolution
2.74 Å
R-value
0.222
Predicted activity confidence0.7%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
STPTINIPASPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPAAIILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMEVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALETA
UniProt reference sequence
SPFMQKLGFGTGVNVYLMKRSPRGLSHSPWAVKKINPICNDHYRSVYQKRLMDEAKILKSLHHPNIVGYRAFTEANDGSLCLAMEYGGEKSLNDLIEERYKASQDPFPAAIILKVALNMARGLKYLHQEKKLLHGDIKSSNVVIKGDFETIKICDVGVSLPLDENMTVTDPEACYIGTEPWKPKEAVEENGVITDKADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDFDDEAYYAALGTRPPINMEELDESYQKVIELFSVCTNEDPKDRPSAAHIVEALET
Aligned reference sequence
SPFM----------------QKLGFGT----------------GVNVYLMKR--SPRGL---------------------------------SHSPWAVKKIN--PICNDHYRSVY-----------------------QKRLMDEAKILKSL--------------------------HHPNIVGYRAFTEAND--G-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SLCLAMEYGGE--------------KSLNDLIEE--RYKASQD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFPAAIILKVALNMARGLKYLHQE--K------------------------------------------KLLHGDI-----KSSNVVIKG--D-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FETIKICDVGVSLPLDENMTV--TDPE------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACYIGTEPWKPKEAVEE--N-----------------------------GVIT-DKADIFAFGLTLWEMMTL-----------------SIPHIN--LSNDDDDEDKTFDESD--------------------------------FDDEAYYAALGTR--PPINMEELDESY---------------------------------------------------------------------------------------------------QKVIELFSVCTNEDPKDRPS--------------AAHIVEALET
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
SPFM----------------QKLGFGT----------------GVNVYLMKR--SPRGL---------------------------------SHSPWAVKKIN--PICNDHYRSVY-----------------------QKRLMDEAKILKSL--------------------------HHPNIVGYRAFTEAND--G-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SLCLAMEYGGE--------------KSLNDLIEE--RYKASQD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFPAAIILKVALNMARGLKYLHQE--K------------------------------------------KLLHGDI-----KSSNVVIKG--D-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FETIKICDVGVSLPLDENMEV--TDPE------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACYIGTEPWKPKEAVEE--N-----------------------------GVIT-DKADIFAFGLTLWEMMTL-----------------SIPHIN--LSNDDDDEDKTFDESD--------------------------------FDDEAYYAALGTR--PPINMEELDESY---------------------------------------------------------------------------------------------------QKVIELFSVCTNEDPKDRPS--------------AAHIVEALET
Activation segment
DVGVSLPLDENMEV--TDPE------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACYIGTEPWKPKE
Binding pocket
QKLGFGTGVNVYLWAVKKIRLMDEAKILKSLHPNIVGYRAFCLAMEYGGEKSLNDLIEELHQEKKLLHGDIKSSNVVIICDVGVS
Ligand info
Orthosteric ligand
None
Allosteric ligand
HC4
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5J0A, Chain A