5HVJ Chain B
LIM domain kinase 1 (LIMK1)
Active — 59.6%DFG-inαC-in
Resolution
2.2 Å
R-value
0.208
Predicted activity confidence59.6%
Structure info
Alternate conformationB
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
PHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRNLNSHNCLVRENKNVVVADFGLARLVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGES
UniProt reference sequence
LIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLET
Aligned reference sequence
LIHG----------------EVLGKGC----------------FGQAIKVTH--RE------------------------------------TGEVMVMKELI--RFDEET----------------------------QRTFLKEVKVMRCL--------------------------EHPNVLKFIGVLYKDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLNFITEYIKG--------------GTLRGIIKS--MDS------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QYPWSQRVSFAKDIASGMAYLHSM---------------------------------------------NIIHRDL-----NSHNCLVRE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKNVVVADFGLARLMVDEKTQ--PEGLRSLKKPDRKKR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVVGNPYWMAPEMING--------------------------------RSYD-EKVDVFSFGIVLCEIIGR-------------------------VNADPDYLPRTM------------------------------------DFGLNVRGFLDRY--CPPNCP---------------------------------------------------------------------------------------------------------PSFFPITVRCCDLDPEKRPS--------------FVKLEHWLET
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LIHG----------------EVLGKGC----------------FGQAIKVTH--RE------------------------------------TGEVMVMKELI--RFDEET----------------------------QRTFLKEVKVMRCL--------------------------EHPNVLKFIGVLYKDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLNFITEYIKG--------------GTLRGIIKS--MDS------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QYPWSQRVSFAKDIASGMAYLHSM---------------------------------------------NIIHRNL-----NSHNCLVRE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKNVVVADFGLARL---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VVGNPYWMAPEMING--------------------------------RSYD-EKVDVFSFGIVLCEIIGR-------------------------VNADPDYLPRTM------------------------------------DFGLNVRGFLDRY--CPPNCP---------------------------------------------------------------------------------------------------------PSFFPITVRCCDLDPEKRPS--------------FVKLEHWLET
Activation segment
DFGLARL---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VVGNPYWMAPE
Binding pocket
EVLGKGCFGQAIKMVMKELTFLKEVKVMRCLEPNVLKFIGVNFITEYIKGGTLRGIIKSYLHSMNIIHRNLNSHNCLVVADFGLA
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5HVJ, Chain B