5DE2 Chain B
Serine/threonine-protein kinase Nek7 (NEK7)
Inactive — 0.0%DFG-outαC-out
Resolution
2.78 Å
R-value
0.208
Predicted activity confidence0.0%
Structure info
Alternate conformationB
Missing atoms9
Missing residues3
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
KALRPDMGYNTLANFRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKFYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTA
UniProt reference sequence
FRIEKKIGRGQFSEVYRAACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALEHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKR
Aligned reference sequence
FRIE----------------KKIGRGQ----------------FSEVYRAAC--LL------------------------------------DGVPVALKKVQ--IFDLMDAKA-------------------------RADCIKEIDLLKQL--------------------------NHPNVIKYYASFIEDN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELNIVLELADA--------------GDLSRMIKH--FKKQKR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LIPERTVWKYFVQLCSALEHMHSR---------------------------------------------RVMHRDI-----KPANVFITA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGVVKLGDLGLGRFFSSKTTA--A---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HSLVGTPYYMSPERIHE--------------------------------NGYN-FKSDIWSLGCLLYEMAAL-----------------QSPFYG--DK----------------------------------------------MNLYSLCKKIEQC--DYPPLPSDHYS----------------------------------------------------------------------------------------------------EELRQLVNMCINPDPEKRPD--------------VTYVYDVAKR
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FRIE----------------KKIGRGQ----------------FSEVYRAAC--LL------------------------------------DGVPVALKKVQ--IFDLMDAKA-------------------------RADCIKEIDLLKQL--------------------------NHPNVIKFYASFIEDN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELNIVLELADA--------------GDLSRMIKH--FKKQKR---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LIPERTVWKYFVQLCSALEHMHSR---------------------------------------------RVMHRDI-----KPANVFITA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGVVKLGDL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PYYMSPERIHE--------------------------------NGYN-FKSDIWSLGCLLYEMAAL-----------------QSPFYG--DK----------------------------------------------MNLYSLCKKIEQC--DYPPLPSDHYS----------------------------------------------------------------------------------------------------EELRQLVNMCINPDPEKRPD--------------VTYVYDVAKR
Activation segment
DL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PYYMSPE
Binding pocket
KKIGRGQFSEVYRVALKKVDCIKEIDLLKQLNPNVIKFYASNIVLELADAGDLSRMIKHHMHSRRVMHRDIKPANVFILGDL___
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationout
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5DE2, Chain B