5DBX Chain A
STE20/SPS1-related proline-alanine-rich protein kinase (STK39)
Inactive — 1.0%DFG-inαC-outATPlike · ANP
Resolution
2.5 Å
R-value
0.208
Predicted activity confidence1.0%
Structure info
Alternate conformationA
Missing atoms0
Missing residues1
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
AVGWPIRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLAFVGTPWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKKFFQKAKNREYLIEKLLTR
UniProt reference sequence
YELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFF
Aligned reference sequence
YELQ----------------EVIGSGA----------------TAVVQAALC--KP------------------------------------RQERVAIKRIN--LEKCQTS---------------------------MDELLKEIQAMSQC--------------------------SHPNVVTYYTSFVVKD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELWLVMKLLSG--------------GSMLDIIKY--IVNRGEHKNG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VLEEAIIATILKEVLEGLDYLHRN---------------------------------------------GQIHRDL-----KAGNILLGE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGSVQIADFGVSAFLATGGDV--TRNKVR----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTFVGTPCWMAPEVMEQ--V-----------------------------RGYD-FKADMWSFGITAIELATG-----------------AAPYHK--------------------------------------------------YPPMKVLMLTLQN--DPPTLETGVEDKEMMKKYG--------------------------------------------------------------------------------------------KSFRKLLSLCLQKDPSKRPT--------------AAELLKCKFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YELQ----------------EVIGSGA----------------TAVVQAALC--KP------------------------------------RQERVAIKRIN--LEKCQTS---------------------------MDELLKEIQAMSQ---------------------------SHPNVVTYYTSFVVKD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELWLVMKLLSG--------------GSMLDIIKY--IVNRGEHKNG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VLEEAIIATILKEVLEGLDYLHRN---------------------------------------------GQIHRDL-----KAGNILLGE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGSVQIADFGVSAFLA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FVGTP-WMAPEVMEQ--V-----------------------------RGYD-FKADMWSFGITAIELATG-----------------AAPYHK--------------------------------------------------YPPMKVLMLTLQN--DPPTLETGVEDKEMMKKYG--------------------------------------------------------------------------------------------KSFRKLLSLCLQKDPSKRPT--------------AAELLK-KFF
Activation segment
DFGVSAFLA-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FVGTP-WMAPE
Binding pocket
EVIGSGATAVVQAVAIKRIELLKEIQAMSQ_SPNVVTYYTSWLVMKLLSGGSMLDIIKYYLHRNGQIHRDLKAGNILLIADFGVS
Ligand info
Orthosteric ligand
ANP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5DBX, Chain A