5D9H Chain A
STE20/SPS1-related proline-alanine-rich protein kinase (STK39)
Inactive — 0.6%DFG-inαC-outATPlike · ATP
Resolution
3.1 Å
R-value
0.24
Predicted activity confidence0.6%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
AVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLL
UniProt reference sequence
YELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFF
Aligned reference sequence
YELQ----------------EVIGSGA----------------TAVVQAALC--KP------------------------------------RQERVAIKRIN--LEKCQTS---------------------------MDELLKEIQAMSQC--------------------------SHPNVVTYYTSFVVKD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELWLVMKLLSG--------------GSMLDIIKY--IVNRGEHKNG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VLEEAIIATILKEVLEGLDYLHRN---------------------------------------------GQIHRDL-----KAGNILLGE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGSVQIADFGVSAFLATGGDV--TRNKVR----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTFVGTPCWMAPEVMEQ--V-----------------------------RGYD-FKADMWSFGITAIELATG-----------------AAPYHK--------------------------------------------------YPPMKVLMLTLQN--DPPTLETGVEDKEMMKKYG--------------------------------------------------------------------------------------------KSFRKLLSLCLQKDPSKRPT--------------AAELLKCKFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YELQ----------------EVIGSGA----------------TAVVQAALC--KP------------------------------------RQERVAIKRIN--LEKCQTS---------------------------MDELLKEIQAMSQC--------------------------SHPNVVTYYTSFVVKD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELWLVMKLLSG--------------GSMLDIIKY--IVNRGEHKNG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VLEEAIIATILKEVLEGLDYLHRN---------------------------------------------GQIHRDL-----KAGNILLGE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGSVQIADFGVSAFLATGGDV--TR-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VGTPCWMAPEVMEQ--V-----------------------------RGYD-FKADMWSFGITAIELATG-----------------AAPYHK--------------------------------------------------YPPMKVLMLTLQN--DPPTLETGV--KEMMKKYG--------------------------------------------------------------------------------------------KSFRKLLSLCLQKDPSKRPT--------------AAELLKCKFF
Activation segment
DFGVSAFLATGGDV--TR-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VGTPCWMAPE
Binding pocket
EVIGSGATAVVQAVAIKRIELLKEIQAMSQCSPNVVTYYTSWLVMKLLSGGSMLDIIKYYLHRNGQIHRDLKAGNILLIADFGVS
Ligand info
Orthosteric ligand
ATP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 5D9H, Chain A