4YUR Chain A
Serine/threonine-protein kinase PLK4 (PLK4)
Inactive — 0.8%DFG-inαC-outType1 · 4J7
Resolution
2.65 Å
R-value
0.202
Predicted activity confidence0.8%
Structure info
Alternate conformation—
Missing atoms33
Missing residues2
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
IGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLESDVWSLGCMFYTLLIGRPPFTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMS
UniProt reference sequence
FKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFM
Aligned reference sequence
FKVG----------------NLLGKGS----------------FAGVYRAES--IH------------------------------------TGLEVAIKMID--KKAMYKAGM-------------------------VQRVQNEVKIHCQL--------------------------KHPSILELYNYFEDSN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YVYLVLEMCHN--------------GEMNRYLKN--RVK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFSENEARHFMHQIITGMLYLHSH---------------------------------------------GILHRDL-----TLSNLLLTR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NMNIKIADFGLATQLKMPHEK--H---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTLCGTPNYISPEIATR--------------------------------SAHG-LESDVWSLGCMFYTLLIG-----------------RPPFDT--------------------------------------------------DTVKNTLNKVVLA--DYEMPSFLS------------------------------------------------------------------------------------------------------IEAKDLIHQLLRRNPADRLS--------------LSSVLDHPFM
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FKVG----------------NLLGKGS----------------FAGVYRAES--IH------------------------------------TGLEVAIKMID--KKAMYKAGM-------------------------VQRVQNEVKIHCQL--------------------------KHPSILELYNYFEDSN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YVYLVLEMCHN--------------GEMNRYLKN--RVK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFSENEARHFMHQIITGMLYLHSH---------------------------------------------GILHRDL-----TLSNLLLTR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NMNIKIADF----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------G-LESDVWSLGCMFYTLLIG-----------------RPPF-----------------------------------------------------TVKNTLNKVVLA--DYEMPSFLS------------------------------------------------------------------------------------------------------IEAKDLIHQLLRRNPADRLS--------------LSSVLDHPFM
Activation segment
DF-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Binding pocket
NLLGKGSFAGVYRVAIKMIRVQNEVKIHCQLKPSILELYNYYLVLEMCHNGEMNRYLKNYLHSHGILHRDLTLSNLLLIADFG__
Ligand info
Orthosteric ligand
4J7
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4YUR, Chain A