4YHJ Chain B
G protein-coupled receptor kinase 4 (GRK4)
Active — 98.7%DFG-inαC-inATPlike · AN2
Resolution
2.6 Å
R-value
0.2
Predicted activity confidence98.7%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
GRSKKWKEILTLPPVSQCSELRHSIEKDYSSLCDKQPIGRRLFRQFCDTKPTLKRHIEFLDAVAEYEVADDEDRSDCGLSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSKKAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLGNPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCGIYLDTADEDFYARFATGCVSIPWQNEMIESGCFKDINK
UniProt reference sequence
FRHYRVLGKGGFGEVCACQVRATGKMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLGNPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTVGYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSICRMLLTKNPSKRLGCRGEGAAGVKQHPVF
Aligned reference sequence
FRHY----------------RVLGKGG----------------FGEVCACQV--RA------------------------------------TGKMYACKKLQ--KKRIKKRKG-------------------------EAMALNEKRILEKV--------------------------QSRFVVSLAYAYETKD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALCLVLTIMNG--------------GDLKFHIYN--LGNP-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GFDEQRAVFYAAELCCGLEDLQRE---------------------------------------------RIVYRDL-----KPENILLDD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGHIRISDLGLATEIPEGQRV------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGRVGTVGYMAPEVVNN--------------------------------EKYT-FSPDWWGLGCLIYEMIQG-----------------HSPFKK--YKEK--------------------------------------------VKWEEVDQRIKND--TEEYSEKFS------------------------------------------------------------------------------------------------------EDAKSICRMLLTKNPSKRLG--CRGEG-------AAGVKQHPVF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FRHY----------------RVLGKGG----------------FGEVCACQV--RA------------------------------------TGKMYACKKLQ--KKRIKKRKG-------------------------EAMALNEKRILEKV--------------------------QSRFVVSLAYAYETKD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALCLVLTIMNG--------------GDLKFHIYN--LGNP-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GFDEQRAVFYAAELCCGLEDLQRE---------------------------------------------RIVYRDL-----KPENILLDD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGHIRISDLGLATEIPEGQRV------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGRVGTVGYMAPEVVNN--------------------------------EKYT-FSPDWWGLGCLIYEMIQG-----------------HSPFKK--YKEK--------------------------------------------VKWEEVDQRIKND--TEEYSEKFS------------------------------------------------------------------------------------------------------EDAKSICRMLLTKNPSKRLG--CRGEG-------AAGVKQHPVF
Activation segment
DLGLATEIPEGQRV------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RGRVGTVGYMAPE
Binding pocket
RVLGKGGFGEVCAYACKKLMALNEKRILEKVQRFVVSLAYACLVLTIMNGGDLKFHIYNDLQRERIVYRDLKPENILLISDLGLA
Ligand info
Orthosteric ligand
AN2
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4YHJ, Chain B