4Y83 Chain B
Mitogen-activated protein kinase kinase kinase 8 (MAP3K8)
Active — 98.9%DFG-inαC-inType1 · 49B
Resolution
2.89 Å
R-value
0.204
Predicted activity confidence98.9%
Structure info
Alternate conformation—
Missing atoms43
Missing residues2
Salt bridge (KinCore)Saltbr-na
Sequence info
PDB sequence
LSSVRYGTVEDLLAFANHISPQESGILLNMVITPQNGRYQIDSDVLLIPWKLTYRNIGSDFIPRFGKVYLAQDIKTKKRMACKLIPVDQFKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVQMTEDVYFPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPPSYLYIIHKQAPPLEDIADDCSPGMRELIEASLERNPNHRPRAADLLKHEALNP
UniProt reference sequence
VLLIPWKLTYRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKLIPVDQFKPSDVEIQACFRHENIAELYGAVLWGETVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHVLKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVQMTEDVYFPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIADDCSPGMRELIEASLERNPNHRPRAADLLKHEAL
Aligned reference sequence
VLLI--PWKLTYRNIGS------------DFIPRGA-------FGKVYLAQD--IK------------------------------------TKKRMACKLIP--V---------------------------------DQFKPSDVEIQACF--------------------------RHENIAELYGAVLWGE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TVHLFMEAGEG--------------GSVLEKLES--CG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PMREFEIIWVTKHVLKGLDFLHSK---------------------------------------------KVIHHDI-----KPSNIVFM---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STKAVLVDFGLSVQMTEDVYF--P---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KDLRGTEIYMSPEVILC--------------------------------RGHS-TKADIYSLGATLIHMQTG-----------------TPPWVK--RYPR--------------------------------------------SAYPSYLYIIHKQ--APPLEDIADDCS---------------------------------------------------------------------------------------------------PGMRELIEASLERNPNHRPR--------------AADLLKHEAL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
VLLI--PWKLTYRNIGS------------DFIPR---------FGKVYLAQD--IK------------------------------------TKKRMACKLIP--V---------------------------------DQFKPSDVEIQACF--------------------------RHENIAELYGAVLWGE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TVHLFMEAGEG--------------GSVLEKLES--CG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PMREFEIIWVTKHVLKGLDFLHSK---------------------------------------------KVIHHDI-----KPSNIVFM---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STKAVLVDFGLSVQMTEDVYF--P---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KDLRGTEIYMSPEVILC--------------------------------RGHS-TKADIYSLGATLIHMQTG-----------------TPPWVK--RYP------------------------------------------------PSYLYIIHKQ--APPLEDIADDCS---------------------------------------------------------------------------------------------------PGMRELIEASLERNPNHRPR--------------AADLLKHEAL
Activation segment
DFGLSVQMTEDVYF--P---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KDLRGTEIYMSPE
Binding pocket
DFIPR__FGKVYLMACKLIFKPSDVEIQACFRENIAELYGAHLFMEAGEGGSVLEKLESFLHSKKVIHHDIKPSNIVFLVDFGLS
Ligand info
Orthosteric ligand
49B
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4Y83, Chain B