4XJ0 Chain B
Mitogen-activated protein kinase 1 (MAPK1)
Active — 99.8%DFG-inαC-inType1 · 41B
Resolution
2.58 Å
R-value
0.21
Predicted activity confidence99.8%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
EMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLEQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPG
UniProt reference sequence
YTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYL
Aligned reference sequence
YTNL----------------SYIGEGA----------------YGMVCSAYD--NV------------------------------------NKVRVAIKKIS--PFEHQTY---------------------------CQRTLREIKILLRF--------------------------RHENIIGINDIIRAPT--IEQMK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYIVQDLME---------------TDLYKLLKT--Q--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HLSNDHICYFLYQILRGLKYIHSA---------------------------------------------NVLHRDL-----KPSNLLLNT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCDLKICDFGLARVADPDHDH--TGFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TEYVATRWYRAPEIMLN--S-----------------------------KGYT-KSIDIWSVGCILAEMLSN-----------------RPIFPG--------------------------------------------------KHYLDQLNHILGI--LGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNAD-----------------------------------------------------------------------SKALDLLDKMLTFNPHKRIE--------------VEQALAHPYL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YTNL----------------SYIGEGA----------------YGMVCSAYD--NV------------------------------------NKVRVAIKKIS--PFEHQTY---------------------------CQRTLREIKILLRF--------------------------RHENIIGINDIIRAPT--IEQMK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYIVQDLME---------------TDLYKLLKT--Q--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HLSND-ICYFLYQILRGLKYIHSA---------------------------------------------NVLHRDL-----KPSNLLLNT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCDLKICDFGLARVADPDHDH--TGFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TEYVATRWYRAPEIMLN--S-----------------------------KGYT-KSIDIWSVGCILAEMLSN-----------------RPIFPG--------------------------------------------------KHYLDQLNHILGI--LGSPSQED-------KARNYLLSLPHKNKVPWNRLFPNAD-----------------------------------------------------------------------SKALDLLDKMLTFNPHKRIE--------------VEQALAHPYL
Activation segment
DFGLARVADPDHDH--TGFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TEYVATRWYRAPE
Binding pocket
SYIGEGAYGMVCSVAIKKIRTLREIKILLRFRENIIGINDIYIVQDLME_TDLYKLLKTYIHSANVLHRDLKPSNLLLICDFGLA
Ligand info
Orthosteric ligand
41B
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4XJ0, Chain B