4UYA Chain A
Mitogen-activated protein kinase kinase kinase 21 (MAP3K21)
Inactive — 0.1%DFG-inαC-outATPlike · AGS
Resolution
2.8 Å
R-value
0.204
Predicted activity confidence0.1%
Structure info
Alternate conformation—
Missing atoms52
Missing residues7
Salt bridge (KinCore)Saltbr-none
Sequence info
PDB sequence
SPVHVAFERLELKELIGAGQVYRATWQGQEVAVKAAAAESVRREARLFAMLRHPNIIELRGVCLQQPHLCLVLEFARGGALNRALAGRRIPPHVLVNWAVQIARGMLYLHEEAFVPILHRDLKSSNILLLEKIEHDICNKTLKITDFGAYAWMAPEVIKSSLFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTCPEPFAKLMKECWQQDPHIRPSFALILEQLTAMTEMPQESFHSMQDDWKLEIQQMFDELRTKEK
UniProt reference sequence
LELKELIGAGGFGQVYRATWQGQEVAVKAARQDPEQDAAAAAESVRREARLFAMLRHPNIIELRGVCLQQPHLCLVLEFARGGALNRALAAANAAPDPRAPGPRRARRIPPHVLVNWAVQIARGMLYLHEEAFVPILHRDLKSSNILLLEKIEHDDICNKTLKITDFGLAREWHRTTKMSTAGTYAWMAPEVIKSSLFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTCPEPFAKLMKECWQQDPHIRPSFALILEQLTA
Aligned reference sequence
LELK----------------ELIGAGG----------------FGQVYRATW----------------------------------------QGQEVAVKAAR--QDPEQDAAAA------------------------AESVRREARLFAML--------------------------RHPNIIELRGVCLQQP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HLCLVLEFARG--------------GALNRALAA--ANAAPDPRAPGPRRAR-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RIPPHVLVNWAVQIARGMLYLHEE--AFV----------------------------------------PILHRDL-----KSSNILLLE--KIEHDDIC----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKTLKITDFGLAREWHRTTK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MSTAGTYAWMAPEVIKS--------------------------------SLFS-KGSDIWSYGVLLWELLTG-----------------EVPYRG--------------------------------------------------IDGLAVAYGVAVN--KLTLPIPSTCP----------------------------------------------------------------------------------------------------EPFAKLMKECWQQDPHIRPS--------------FALILEQLTA
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LELK----------------ELIGA-------------------GQVYRATW----------------------------------------QGQEVAVK------------AAA------------------------AESVRREARLFAML--------------------------RHPNIIELRGVCLQQP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HLCLVLEFARG--------------GALNRALA-----------------GR-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RIPPHVLVNWAVQIARGMLYLHEE--AFV----------------------------------------PILHRDL-----KSSNILLLE--KIEH-DIC----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKTLKITDF----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAYAWMAPEVIKS--------------------------------SLFS-KGSDIWSYGVLLWELLTG-----------------EVPYRG--------------------------------------------------IDGLAVAYGVAVN--KLTLPIPSTCP----------------------------------------------------------------------------------------------------EPFAKLMKECWQQDPHIRPS--------------FALILEQLTA
Activation segment
DF----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GAYAWMAPE
Binding pocket
ELIGAG___QVYRVAVK__SVRREARLFAMLRPNIIELRGVCLVLEFARGGALNRALA_EEAFVPILHRDLKSSNILLITDFG_A
Ligand info
Orthosteric ligand
AGS
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4UYA, Chain A