4TPT Chain B
LIM domain kinase 2 (LIMK2)
Inactive — 0.0%DFG-outαC-outType3 · 35H
Resolution
2.6 Å
R-value
0.223
Predicted activity confidence0.0%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
DLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGL
UniProt reference sequence
LIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEA
Aligned reference sequence
LIHG----------------EVLGKGF----------------FGQAIKVTH--KA------------------------------------TGKVMVMKELI--RCDEET----------------------------QKTFLTEVKVMRSL--------------------------DHPNVLKFIGVLYKDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLNLLTEYIEG--------------GTLKDFLRS--MD-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFPWQQKVRFAKGIASGMAYLHSM---------------------------------------------CIIHRDL-----NSHNCLIKL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKTVVVADFGLSRLIVEERKR--APMEKATTKKRTLRKNDRKKR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVVGNPYWMAPEMLNG--------------------------------KSYD-ETVDIFSFGIVLCEIIGQ-------------------------VYADPDCLPRTL------------------------------------DFGLNVKLFWEKF--VPTDCP---------------------------------------------------------------------------------------------------------PAFFPLAAICCRLEPESRPA--------------FSKLEDSFEA
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LIHG----------------EVLGKGF----------------FGQAIKVTH--KA------------------------------------TGKVMVMKELI--RCDEET----------------------------QKTFLTEVKVMRSL--------------------------DHPNVLKFIGVLYKDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLNLLTEYIEG--------------GTLKDFLRS--MD-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PFPWQQKVRFAKGIASGMAYLHSM---------------------------------------------CIIHRDL-----NSHNCLIKL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKTVVVADFGLSRLIV-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVVGNPYWMAPEMLNG--------------------------------KSYD-ETVDIFSFGIVLCEIIGQ-------------------------VYADPDCLPRTL------------------------------------DFGLNVKLFWEKF--VPTDCP---------------------------------------------------------------------------------------------------------PAFFPLAAICCRLEPESRPA--------------FSKLEDSFEA
Activation segment
DFGLSRLIV-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTVVGNPYWMAPE
Binding pocket
EVLGKGFFGQAIKMVMKELTFLTEVKVMRSLDPNVLKFIGVNLLTEYIEGGTLKDFLRSYLHSMCIIHRDLNSHNCLIVADFGLS
Ligand info
Orthosteric ligand
35H
Allosteric ligand
None
Ligand typeType3
Consensus conformation
DFG conformationout
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4TPT, Chain B