4REW Chain A
5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1)
Active — 99.9%DFG-inαC-inType1 · STU
Resolution
4.58 Å
R-value
0.234
Predicted activity confidence99.9%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
GSVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRASIKDIREHEWFKQDLPKYLFPEIDNRRIMNEAKDFYLATSPPDHHLTRPHPERVPFLVAETRKAKWHLGIRSQSRPNDIMAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDGPRPGSHTIEFFEMCANLIKILAQ
UniProt reference sequence
YILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWF
Aligned reference sequence
YILG----------------DTLGVGT----------------FGKVKVGKH--EL------------------------------------TGHKVAVKILN--RQKIRSLDV-------------------------VGKIRREIQNLKLF--------------------------RHPHIIKLYQVISTPS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DIFMVMEYVSG--------------GELFDYICK--NG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLDEKESRRLFQQILSGVDYCHRH---------------------------------------------MVVHRDL-----KPENVLLDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HMNAKIADFGLSNMMSDGEFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RTSCGSPNYAAPEVISG--------------------------------RLYAGPEVDIWSSGVILYALLCG-----------------TLPFDD--------------------------------------------------DHVPTLFKKICDG--IFYTPQYLN------------------------------------------------------------------------------------------------------PSVISLLKHMLQVDPMKRAT--------------IKDIREHEWF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YILG----------------DTLGVGT----------------FGKVKVGKH--EL------------------------------------TGHKVAVKILN--RQKIRSLDV-------------------------VGKIRREIQNLKLF--------------------------RHPHIIKLYQVISTPS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DIFMVMEYVSG--------------GELFDYICK--NG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLDEKESRRLFQQILSGVDYCHRH---------------------------------------------MVVHRDL-----KPENVLLDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HMNAKIADFGLSNMMSDGEFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RTSCGSPNYAAPEVISG--------------------------------RLYAGPEVDIWSSGVILYALLCG-----------------TLPFDD--------------------------------------------------DHVPTLFKKICDG--IFYTPQYLN------------------------------------------------------------------------------------------------------PSVISLLKHMLQVDPMKRAS--------------IKDIREHEWF
Activation segment
DFGLSNMMSDGEFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RTSCGSPNYAAPE
Binding pocket
DTLGVGTFGKVKVVAVKILKIRREIQNLKLFRPHIIKLYQVFMVMEYVSGGELFDYICKYCHRHMVVHRDLKPENVLLIADFGLS
Ligand info
Orthosteric ligand
STU
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4REW, Chain A