4OTI Chain A
Serine/threonine-protein kinase N1 (PKN1)
Inactive — 0.2%DFG-inαC-inType1 · MI1
Resolution
1.93 Å
R-value
0.187
Predicted activity confidence0.2%
Structure info
Alternate conformationB
Missing atoms0
Missing residues1
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
RKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLPPRDARPLTAAEQAAFLDFDFVAG
UniProt reference sequence
FKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFF
Aligned reference sequence
FKFL----------------AVLGRGH----------------FGKVLLSEF--RP------------------------------------SGELFAIKALK--KGDIVARDE-------------------------VESLMCEKRILAAV--TSA---------------------GHPFLVNLFGCFQTPE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HVCFVMEYSAG--------------GDLMLHIHS--D--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VFSEPRAIFYSACVVLGLQFLHEH---------------------------------------------KIVYRDL-----KLDNLLLDT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGYVKIADFGLCKEGMGYGDR--T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STFCGTPEFLAPEVLTD--------------------------------TSYT-RAVDWWGLGVLLYEMLVG-----------------ESPFPG--------------------------------------------------DDEEEVFDSIVND--EVRYPRFLS------------------------------------------------------------------------------------------------------AEAIGIMRRLLRRNPERRLG--SSERD-------AEDVKKQPFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FKFL----------------AVLGRGH----------------FGKVLLSEF--RP------------------------------------SGELFAIKALK--KGDIVARDE-------------------------VESLMCEKRILAAV--TSA---------------------GHPFLVNLFGCFQTPE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HVCFVMEYSAG--------------GDLMLHIHS--D--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VFSEPRAIFYSACVVLGLQFLHEH---------------------------------------------KIVYRDL-----KLDNLLLDT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGYVKIADFGL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------T-RAVDWWGLGVLLYEMLVG-----------------ESPFPG--------------------------------------------------DDEEEVFDSIVND--EVRYPRFLS------------------------------------------------------------------------------------------------------AEAIGIMRRLLRRNPERRLG--SSERD-------AEDVKKQPFF
Activation segment
DFGL-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Binding pocket
AVLGRGHFGKVLLFAIKALSLMCEKRILAAVTPFLVNLFGCCFVMEYSAGGDLMLHIHSFLHEHKIVYRDLKLDNLLLIADFGL_
Ligand info
Orthosteric ligand
MI1
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4OTI, Chain A